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Plasmid#194901PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.7
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA
Plasmid#186408PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pA-RFP-rG2
Plasmid#188969PurposeIPTG inducible mCherry with sgRNADepositorInsertsmCherry
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2
Plasmid#188964PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus
Plasmid#186409PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA
Plasmid#186410PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_ReBphP-PCM_IRES_sfGFP
Plasmid#166917PurposeExpress ReBphP-PCM and sfGFPDepositorInsertsReBphP-PCM
sfGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
UseTagsExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_RhoB
Plasmid#65698PurposeExpresses ONL-RhoB in mammalian cellsDepositorInsertRhoB (RHOB Human)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_RhoB
Plasmid#65696PurposeExpresses YNL-RhoB in mammalian cellsDepositorInsertRhoB (RHOB Human)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_vinculin
Plasmid#65691PurposeExpresses Vinculin-CNL in mammalian cellsDepositorInsertvinculin (Vcl Mouse)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_vinculin
Plasmid#65692PurposeExpresses Vinculin-ONL in mammalian cellsDepositorInsertvinculin (Vcl Mouse)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_vinculin
Plasmid#65690PurposeExpresses Vinculin-YNL in mammalian cellsDepositorInsertvinculin (Vcl Mouse)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_ITPKA
Plasmid#65689PurposeExpresses ITPKA-ONL in mammalian cellsDepositorInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsONLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_PTS1
Plasmid#65699PurposeExpresses YNL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_Golgi
Plasmid#65694PurposeExpresses Golgi-CNL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_TUBB5
Plasmid#65679PurposeExpresses beta-tubulin-CNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_Golgi
Plasmid#65693PurposeExpresses Golgi-YNL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only