-
Plasmid#128231PurposeC1824U mutation Lamin A splicing reporterDepositorInsertlamin A (LMNA Human)
UseTagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationC1824UPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI AscI MR1 res
Plasmid#214752Purposeexpresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cellsDepositorInsertMHC class I-related protein 1 (MR1 Human)
UseTagsIRES eGFPExpressionMammalianMutationsilent mutations to prevent editing by CRISPR/Cas…PromoterCMVAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-KIF1B beta-600-1400
Plasmid#79109PurposeMammalian expression of GFP-KIF1B beta-600-1400DepositorInsertGFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
UseTagsGFPExpressionMammalianMutationexon 25 missing (natural splice varient)PromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorUseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2
Plasmid#40978DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 S310F
Plasmid#40991DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationS310FPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 A775_G776insYVMA
Plasmid#40982DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationA775_G776insYVMAPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 S310Y
Plasmid#40992DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationS310YPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 G309E
Plasmid#40990DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationG309EPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 C311R
Plasmid#40980DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationC311RPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 C311S
Plasmid#40985DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationC311SPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 E321G
Plasmid#40981DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationE321GPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP2B_LAP
Plasmid#101848PurposeTet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCHMP2B (CHMP2B Human)
UseFlp-frt recombination, tet-inducible expressionTagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationsilent mutation in Thr 104; A to TPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 A775_G776insV, G776C
Plasmid#40979DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationA775_G776insV, G776CPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 D845A
Plasmid#40984DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationD845APromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 C334S
Plasmid#40983DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationC334SPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHis6-SUMO-g0s2
Plasmid#179293PurposeE. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertG0S2 (G0S2 Human)
UseTagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationfull-length G0S2, residues 1-103 from accession n…PromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CAMK1G
Plasmid#20438DepositorInsertCAMK1G (CAMK1G Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only