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Plasmid#65529PurposePuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNMT3b KO Neo
Plasmid#16651DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 3XFlag ATXN1[15Q]-delAXH
Plasmid#33241DepositorInsertATXN1[2Q]-delAXH (ATXN1 Human)
UseTags3xflagExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X
Plasmid#128112PurposeAcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9DepositorInsertAcrX (AcrIIC1 N3F/D15Q/A48I)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyTagsExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
RBM6_RRM_pGEX
Plasmid#135157PurposeRecombinant expression of RBM6 with dual-affinity tagDepositorInsertRBM6 (RBM6 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
Plasmid#61938PurposeCo-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 2-38 dele…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175020PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175018PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEF s57a ED
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_HOIP/RNF31_#6
Plasmid#75252Purposeretroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction controlDepositorInsertshRNA against human RNF31 (RNF31 )
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSuper-PACSIN1-shRNA
Plasmid#72576PurposeExpresses shRNA against PACSIN1DepositorInsertPACSIN1 (Pacsin1 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XL)Cas9
Plasmid#127386PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XL)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
DNMT1 KO Hyg
Plasmid#16648DepositorInsertDNMT1 knock-out construct (DNMT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-EGFP-CLASP2 340-1084
Plasmid#24524DepositorInsertCLASP2 (340-1084) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG mTOR 1968-2549 S2035T
Plasmid#23000DepositorInsertmTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationS2035T aa 1968-2549 onlyPromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pBS
Plasmid#13584DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA3
Plasmid#113132PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE125 XDsh delta COOH-GFP-CS2+
Plasmid#16784DepositorInsertXdsh-delta-COOH (dvl2.L Frog)
UseXenopus expressionTagsGFPExpressionMutationcontains Dsh amino acids 1-588 (deleting 599-736)PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only