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Plasmid#144836PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXB13 (HOXB13 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-UCHL1
Plasmid#22563DepositorInsertUCHL1 (UCHL1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP33
Plasmid#22601DepositorInsertUSP33 (USP33 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mRuby-Keratin-17
Plasmid#55868PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 558, Emission: 605DepositorInsertKeratin (KRT18 Human)
UseTagsmRubyExpressionMammalianMutationV282LPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
GW1-pHRed
Plasmid#31473Purposegenetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeimaDepositorInsertpHRed
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/Lux-nat
Plasmid#120324PurposeBacterial luminescenceDepositorInsertLux operon
UseTagsExpressionBacterialMutationPromoterproDAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
915-Plakophilin 2a-Flag
Plasmid#32230DepositorInsertPlakophilin 2a (PKP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMYH9-WT-V5
Plasmid#183512PurposeGateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.DepositorInsertMYH9 (MYH9 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-hygro-mCherry-RAMP4
Plasmid#118391Purposestable lentiviral expression of cDNADepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6A-EGFR WT
Plasmid#42665DepositorInsertEGFR (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDV10_pCMV-ADAR1-p150
Plasmid#200405PurposeExpresses ADAR1 (p150 isoform) constitutively in mammalian cellsDepositorInsertADAR-p150
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE2
Plasmid#87085PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApolipoprotein E2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2/9 REP-AAP-ΔCAP
Plasmid#205991PurposeRep and AAP protein expression for AAV CREATE librariesDepositorInsertAAV9 Rep and AAP proteins for CREATE libraries
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AGTR2-Tango
Plasmid#66223PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertAGTR2 (AGTR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSBbi-BN
Plasmid#60518PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL2-FLAG
Plasmid#15819DepositorInsertCullin2 (CUL2 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N427PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)
Plasmid#114070PurposeT7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tagDepositorInsertbacteria codon optimized LbCas12a with C-terminal NLS and His-tag
UseTagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-Ef1a-CreN-InteinN
Plasmid#187614PurposeAAV vector that encodes N-terminal portion of split-Intein-mediated split-CreDepositorInsertCreN-InteinN
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-Ef1a-InteinC-CreC
Plasmid#187615PurposeAAV vector encoding C-terminal portion of split-Intein-mediated split-CreDepositorInsertInteinC-CreC
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-NMNAT1
Plasmid#133259PurposeExpress FLAG-tagged NMNAT1 in mammalian cellsDepositorInsertNMNAT1 (NMNAT1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEQ176
Plasmid#83943Purposeexpress beta-galactosidase in eukaryotic cellsDepositorInsertCMV enhancer/promoter
UseTagsbeta-galactosidaseExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
WT-eGFP
Plasmid#215168PurposeEncodes the enhanced green fluorescent proteinDepositorInsertWT-eGFP
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG_ALDOA in pCW57_tTA_Blast
Plasmid#154210PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOADepositorInsertALDOA (ALDOA Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-H6_Streptavidin_sfGFP
Plasmid#124296PurposeHis6-tagged Streptavidin GFP fusion proteinDepositorInsertHis6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
UseTagsHis6 and T7 tagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas ServiceCloning Grade DNAInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-HA-hTAF1
Plasmid#17997DepositorInserthTAF1 (TAF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPath-Violacein-SGE
Plasmid#200003PurposeViolacein pathway. SGEDepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR
Plasmid#204579Purposelentivirus backbone cloning vector and template for barcoded non-neutralizable standard productionDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterTRE3GAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-Intb1
Plasmid#205193PurposeThis vector encodes of a synCAM (Intb1) and GFPDepositorInsertsynCAM Intb1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TU#867 (Punc-119sid-1)
Plasmid#25811DepositorInsertsystemic RNA interference-deficient 1 (sid-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
PX551
Plasmid#60957PurposepAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.DepositorInsertSpCas9
UseAAV and CRISPRTagsHAExpressionMammalianMutationPromoterpMecp2Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP
Plasmid#176015PurposeMammalian expression of EGFP. Parton lab clone DFSDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ZO1-C-14
Plasmid#55165PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorInsertZO1 (TJP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
UseTagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO4A1_STOP
Plasmid#161402PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO4A1 (SLCO4A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
hROSA26 CRISPR-pX330
Plasmid#105927PurposehROSA26 targeting CRISPR plasmidDepositorInserthROSA26 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Xist-PA
Plasmid#26760DepositorInsertXist (Xist Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
UseTagsExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-ATBF1
Plasmid#40927DepositorInsertATBF1 (ZFHX3 Human)
UseTagsFLAGExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-DLL1
Plasmid#205192PurposeThis vector encodes of a synCAM (DLL1) and GFPDepositorInsertsynCAM DLL1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only