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AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Plasmid

#173861

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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vsv-CenpB 1-166-Sgo1-GFP

Plasmid

#108495

Purpose

expression of vsv-CenpB-1-166-Sgo1-EGFP

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHJ425

Plasmid

#20683

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; and change…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
CD8a-f-CTS-EGFP

Plasmid

#86063

Purpose

A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains CD8a luminal and transmembrane domains a…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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ATF4 15: 5’ATF4.uORF1stopm1.GFP

Plasmid

#21862

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stop codon for uORF1 converted to UCG (see author…

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-Tac-TC

Plasmid

#162492

Purpose

Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

partial length Tac (TMD and cytosolic tail) (IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Tac is in partial length with its TMD and cysotol…

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dC

Plasmid

#176466

Purpose

express VPS13D lacking C-terminal 407 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

C-terminal 407 aa is deleted.

Promoter

CMV

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKIF1C-2xFLAG

Plasmid

#130979

Purpose

Expression of KIF1C-2xFLAG in mammalian cells.

Depositor

Anne Straube

Article

Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

Insert

KIF1C-2xFLAG (KIF1C Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

4 silent mutations that make this construct resis…

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pEYFP-hDF C2B

Plasmid

#72583

Purpose

human dysferlin C2B domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2B domain (DYSF Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
FKBP-GTSE1(638-720)-GFP

Plasmid

#100735

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

GTSE1 (GTSE1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

C-terminus only

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmKikGR Vinculin

Plasmid

#105316

Purpose

Photoconvertable focal adhesion protein vinculin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

vinculin (VCL Chicken)

Use

Tags

KikGR

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C2-GFP

Plasmid

#46043

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300A

Plasmid

#62526

Purpose

Serine 300 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to alanine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302A

Plasmid

#62527

Purpose

Serine 302 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to alanine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
emiRFP670-cGAS

Plasmid

#231896

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHsp70(K71E)-GFP

Plasmid

#228196

Purpose

expressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP70(K71E) (HSPA1A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

K71E

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-MLC2 WT

Plasmid

#195235

Purpose

Mammalian expression of GFP tagged MLC2. Wild-type

Depositor

Keith Mostov, David Bryant

Article

Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

Insert

MLC2 (MYL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-INPP5E

Plasmid

#223564

Purpose

Expresses the full-length mouse INPP5E (Pharbin)

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

INPP5E (Inpp5e Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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