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Plasmid#159660PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)
Plasmid#159661PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)
Plasmid#159659PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
7xE-Box::Renilla
Plasmid#124532Purposec-Myc Renilla luciferase reporterDepositorInsertc-Myc Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PyronicSF-mRuby2/pBI-CMV1
Plasmid#124830PurposeHighly resposive GFP-based pyruvate nanosensor.DepositorInsertsPyronicSF
mRuby2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAF112
Plasmid#135920PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseLuciferase; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-NDP52
Plasmid#175749PurposeLentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shLacZ-1735
Plasmid#115454PurposeConstitutive lentiviral expressionDepositorInsertshLacZ-1735
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CABE-T3.1
Plasmid#193267PurposeExpression of CABE-T3.1 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T3.1
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::FLuc
Plasmid#124531PurposeSMAD2 FLuc luciferase reporterDepositorInsertSMAD FLuc reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.46
Plasmid#193284PurposeExpression of CBE-T1.46 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.46
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v1)-Blast
Plasmid#170067PurposeExpresses dCas9 repressor KRAB-dCas9 and blasticidin resistanceDepositorInsertKRAB-dCas9
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRedF
Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationRed mutant S286YPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 IKKE K38A flag
Plasmid#26202DepositorInsertI-kappa-B kinase epsilon K38A (IKBKE Human)
UseTagsFlagExpressionMammalianMutationCatalytic Lysine changed to Alanine (K38A)PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgBFP
Plasmid#192929PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2DepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-metap2
Plasmid#100510PurposeExpresses FLAG tagged metap2 in mammalian cells to make lentivirusDepositorInsertMETAP2 (METAP2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-LSL-nlsGFP-shRNA
Plasmid#175584PurposeConditional express shRNA with control of LoxPDepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GALT-SEP-mRuby3
Plasmid#200938Purposetrans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only