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Plasmid#171944PurposeExpresses INPP4A in mammalian cellsDepositorInsertINPP4A (INPP4A Human)
UseTagsEGFPExpressionMammalianMutationSee Depositor Comments BelowPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV-HIS3
Plasmid#65333Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUsDepositorInsertHIS3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-mCherry
Plasmid#48680PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertNanog (Nanog Mouse)
UseCRISPRTagsCherryExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23A
Plasmid#91138PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
OA984-HA
Plasmid#120362PurposeExpresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporterDepositorInsert1C19 his-tagged single chain antibody
UseUnspecifiedTagsExpressionMutationPromoterAAEL010782 carboxypeptidase A (CPA) gene PromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE
Plasmid#176268PurposeExpression of dCas12f-VPR-mCherry in mammalian cellsDepositorInsertdCas12f
UseLentiviralTagsHA-VPR-mCherryExpressionMammalianMutationD326A, D510APromoterPGKAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
POT2-RFP
Plasmid#65318Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shOrai1
Plasmid#89933PurposeKnockdown human Orai1 expressionDepositorInsertOrai1 shRNA (ORAI1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ensconsin-18-mEos2
Plasmid#99230PurposeMammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.DepositorInsertmEos2
UseTagsEnsconsin (MAP7)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-mTurquoise2-NLS
Plasmid#165443PurposeExpresses nuclear mTurquoise2 from CMV promoterDepositorInsertmTurquoise2-NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Fyn-Fyn Biosensor
Plasmid#124655PurposeFRET biosensor. Fyn-tagged biosensor targeting to membraneDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK402
Plasmid#110598PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21A
Plasmid#91128PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZapLck biosensor
Plasmid#131584PurposeLck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRETDepositorInsertZapLck biosensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK2301
Plasmid#65778PurposeHuman expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNADepositorInsertSt1Cas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2- Empty control -SV40-Luc
Plasmid#26280PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
Lenti-40XUAS-Luc
Plasmid#166455PurposeLuciferase reporterDepositorInsert40XUAS , Luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only