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Plasmid#187454PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-mCherry
Plasmid#129720PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mCherryExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSI-359
Plasmid#131131PurposegRNA expression vector for A-to-G base editing reporterDepositorInsertA-to-G reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1
Plasmid#187458PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSI-535
Plasmid#131130PurposegRNA expression vector for C-to-T base editing reporterDepositorInsertC-to-T reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_sgGFP4
Plasmid#119876PurposegRNA expression vectorDepositorInsertsgGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_sgGFP3
Plasmid#119875PurposegRNA expression vectorDepositorInsertsgGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126644PurposeCloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2 Cryptic PE-Max
Plasmid#216163PurposeExpression of prime editing vector PEMax (Plasmid #174820) but only in cells with TDP-43 loss of functionDepositorInsertPEMax for prime editing with internal cryptic exon
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR63(YUM1)
Plasmid#203336Purposeepisomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR
Plasmid#211820PurposeLentiviral vector expressing a dead GFP adenine base editing reporterDepositorInsertdead GFP reporter
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato
Plasmid#209298PurposeA lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.DepositorInsertsdCas9
tdTomato
UseLentiviralTagsExpressionMammalianMutationPromotertetracycline-inducible promoter (TRE3G)Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 polH aeBlue polH VSV-G
Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3TagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-218
Plasmid#197139PurposeAll-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unitDepositorTypeEmpty backboneUseCRISPRTagsSpCas9-PmCDA1-UGIExpressionMammalianMutationSpCas9(D10A)PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1
Plasmid#187457PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G6 T804N pegRNA (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAP2A-p2A-E2Crimson
Plasmid#83940PurposeLentiviral vector expressing RAP2A-p2A-E2CrimsonDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLC35B2-p2A-E2Crimson
Plasmid#83941PurposeLentiviral vector expressing SLC35B2-p2A-E2CrimsonDepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only