Addgene

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ER-CanlonicSF

Plasmid

#178343

Purpose

To explore endoplasmic reticulum lactate dynamics

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

ER-CanlonicSF

Use

Tags

ER Retention Signal, ER Signal Exon 1, and ER Sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin3DGV

Plasmid

#67886

Purpose

Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the first 53 amino acids of Caveolin3

Promoter

CMV

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only
DF-UAS-sspa-GCaMP6f

Plasmid

#67569

Purpose

Zebrafish expression of a short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

Expression

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
shYES1 # 1

Plasmid

#42546

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-REX-GECO0.9

Plasmid

#61247

Purpose

Expresses REX-GECO0.9 in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Use

Tags

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only
XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

Plasmid

#16754

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-trunc before PDZ (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

truncated before PDZ

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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DF-UAS-spa-GCaMP6f

Plasmid

#67568

Purpose

Zebrafish expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

HIS-tag

Expression

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pPN262

Plasmid

#91607

Purpose

Express sgRNA targeting human EPHX2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

EPHX2 (EPHX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E17A

Plasmid

#157782

Purpose

Expresses a sgRNA targeting stop codon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPN234

Plasmid

#91591

Purpose

Express sgRNA targeting human CYFIP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CYFIP1 (CYFIP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

Plasmid

#106434

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPN013

Plasmid

#91587

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN107

Plasmid

#91636

Purpose

Express sgRNA targeting human KDM4A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KDM4A (KDM4A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN106

Plasmid

#91635

Purpose

Express sgRNA targeting human KDM4A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KDM4A (KDM4A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR - Amplicon, JAK2 sgRNA 2

Plasmid

#70660

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
PDK1 gRNA (BRDN0001145374)

Plasmid

#77008

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDK1 (PDK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPN061

Plasmid

#91594

Purpose

Express sgRNA targeting human CYP26B1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CYP26B1 (CYP26B1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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