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Plasmid#118346PurposeThis plasmid expresses shRNA against hHSF2.DepositorInserthHSF2 shRNA (HSF2 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DYNLL1_Dynein-light
Plasmid#110006PurposeProtein expression and purification of DYNLL1_Dynein-lightDepositorInsertDYNLL1_Dynein-light (DYNLL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 DLC1
Plasmid#24266DepositorUseTagsT7ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-AcGFP-P2A-hBRD4S
Plasmid#197882Purposestudy roles of human BET proteins in human pluripotency reprogrammingDepositorInsertBRD4 (BRD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Grx1
Plasmid#65000PurposeExpression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
Plasmid#194755PurposeLentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-WT
Plasmid#92009PurposeExpresses Lambda-N-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseTagsLambda-N-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHSG1C3
Plasmid#164423Purposesingle guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ctrl)
Plasmid#69215PurposeAdvanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNADepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV promoter
P2A-mNeonGreen
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AchE-CD4d3+4-bio
Plasmid#73119PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bioDepositorInsertAchE (ACHE Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_CELF1
Plasmid#106099PurposeInducible gene expression of FlagHA - CELF1DepositorInsertCELF1 (CELF1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_Giantin_C1
Plasmid#85049PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362
Plasmid#31354DepositorInsertBrd4 1047-1362 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Grx1
Plasmid#64995Purposefor expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophilaDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pf113-bio
Plasmid#47729PurposeExpresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised Pf113
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-dsRedM
Plasmid#73592PurposeExpress human Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6347 MSCV-CMV-Flag-HA-Brd4-444-722
Plasmid#31353DepositorInsertBrd4 444-722 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE
Plasmid#67291PurposeInduce human iN conversion from fibroblastDepositorInsertAsc1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Neurod1-Atoh7HA3
Plasmid#44438DepositorInsertAtoh7-3xHA knock-in (Atoh7 Mouse)
UseCre/Lox and Mouse TargetingTagsNeurod1 left arm, Neurod1 right arm, and loxC2-Re…ExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only