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Plasmid#21576DepositorInsertBmi-1 (Bmi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B3
Plasmid#172844PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mTurquoise2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mTagBFP2-P2A-EGFP
Plasmid#184943PurposeExpresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cellsDepositorInsertmTagBFP2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonTagsExpressionBacterialMutationnoPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2-1
Plasmid#31828DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-CCSS
Plasmid#83424PurposeMouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidues 218 and 219 from C to S (CCSS: TGCTGT -&…PromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK4alpha WT
Plasmid#12187DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTomato-Gamma-Tubulin-17
Plasmid#58099PurposeLocalization: Centrosomes, Excitation: 554, Emission: 581DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPW3755 : HDR-HsIRE1a-10xGS-HaloTag
Plasmid#185677PurposeHDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.DepositorInsertERN1 homology arms (ERN1 Synthetic, Human)
UseCRISPRTagsHaloTagExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn
Plasmid#100947PurposeExpression of Tyrosine Ammonia Lyase from inducible T7 promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HseIF4AII_AC
Plasmid#148439PurposeMammalian Expression of HseIF4AIIDepositorInsertHseIF4AII (EIF4A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-TGAT
Plasmid#84334PurposeExpresses TGAT tagged with mCherryDepositorInsertTGAT (TRIO Human)
UseTagsmCherryExpressionMammalianMutationWTPromoterCMVAvailable sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Tnf
Plasmid#207127PurposeLuciferase vector containing 3'UTR for mouse TnfDepositorInsertTumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K9R
Plasmid#84527PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK(43,61,69,117,240,246,527,582,651)RPromoternoneAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-D261A
Plasmid#31729DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutationChanged D261 to AlaninePromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_HEK4_20-7
Plasmid#201974PurposeExpress a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology armDepositorInsertpegRNA_HEK4_20-7
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127315PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationDeletion of lumenal domainPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR4-huNFATc1nuc
Plasmid#23988DepositorInsertNFATc1 (NFATC1 Human)
UseCloning vectorTagsEE tagExpressionMutationS172A S175A S176A S178A S179A S181A S184A S187A S…PromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hPGK-mCherry-SynaptoZip
Plasmid#177317PurposeLentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseLentiviralTagsMyc and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX-SponGee-Kras
Plasmid#134777Purposemammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertSponGee-Kras
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-RandE3
Plasmid#79870PurposeExpresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.DepositorInsertGFP-RandE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK4mut(K35M)-3XFlag-pBabe
Plasmid#73559Purposevector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tagsDepositorInsertCDK4mut(K35M)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
shCDK4/6(1)
Plasmid#73554Purposevector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)DepositorInsertshRNA against CDK4 + shRNA against CDK6
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX-Lyn-SponGee
Plasmid#134776Purposemammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertLyn-SponGee
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_ABE_D3_empty
Plasmid#203809PurposePlasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).DepositorInsertABE_D3
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo
Plasmid#179439PurposeExpresses rTetR-KRAB(human) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-KRAB
UseTagsExpressionMammalianMutationPromoterpGKAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a1
Plasmid#14538DepositorInsertMKK7a1 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
p1.1-eGFP
Plasmid#162738PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag GCN5 Y621A/P622A
Plasmid#14425DepositorInsertGCN5 Y621A/P622A (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationY621A, P622A. Catalytically inactive acetyltransf…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-st1CAS9-pA
Plasmid#131542PurposeExpresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized st1Cas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
SL016: AAV-CAG-FLEX-CheRiff-GFP
Plasmid#124776PurposeFor AAV based expression of CheRiff in Cre-Positive cellsDepositorInsertCheRiff-GFP
UseAAV and Cre/LoxTagsGFPExpressionMutationPromoterCAGAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_BFP
Plasmid#45297DepositorInsertBFP
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS
Plasmid#79067PurposeExpress owl monkey TRIMCyp in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD8 Flag
Plasmid#74900PurposeExpress COMMD8 in mammalian cellsDepositorInsertCOMMD8 (COMMD8 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNEXdelta-EGFP-ApAF
Plasmid#13335DepositorInsertAplysia activating factor
UseTagsEGFPExpressionMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha
Plasmid#35557DepositorInsertRARalpha (RARA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
XE49 pt Beta-catenin-myc
Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationfour point mutations introduced into the GSK-3 ph…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mCasp8
Plasmid#208782PurposeInducibly expresses mouse caspase8 fused to light-activatable Cry2DepositorInsertmCherry mCaspase8 Cry2 (Casp8 Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag
Plasmid#185684PurposeLow-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationK121YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC55-V5-Flag-His
Plasmid#127819PurposeExpression of human LRRC55 in mammalian cellsDepositorInsertLRRC55 (LRRC55 Human)
UseTagsV5, FLAG, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-E264A
Plasmid#31730DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutationChanged E264 to AlaninePromoterNoneAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha WT
Plasmid#128812PurposeTo make virus expressing Doc2alpha WTDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta WT
Plasmid#128814PurposeTo make virus expressing Doc2beta WTDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB-1753-Nluc I9A/W10A
Plasmid#158604PurposePlasmid expressing KB-1753-Nluc I9A/W10A in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SF-U7snRNA-CypA-E4
Plasmid#190695PurposeControl vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4DepositorInsertU7 snRNA antisense CypA Exon 4
UseLentiviralTagsExpressionMammalianMutationPromoterU7 promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only