Skip to main content
Addgene
Showing: 3631 - 3660 of 4144 results

  2. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229851-plasmid-map-sequence-id-460711

    Enlarge

  3. MZB220

    Plasmid
    #221771
    Purpose
    Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag
    Depositor
    Richard Baker
    Insert
    Arl1 (ARL1 Human)
    Use
    Tags
    CPD
    Expression
    Bacterial
    Mutation
    Q71L
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221771-plasmid-map-sequence-id-455287

    Enlarge

  4. MZB222

    Plasmid
    #221772
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Insert
    ArfGEF1 (ARFGEF1 Human)
    Use
    Tags
    10xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221772-plasmid-map-sequence-id-455976

    Enlarge

  5. L469R USP7 catalytic

    Plasmid
    #227212
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L469R; aa 207-560
    Promoter
    T7-promoter
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227212-plasmid-map-sequence-id-452328

    Enlarge

  6. L299A USP7 catalytic

    Plasmid
    #227213
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L299A; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227213-plasmid-map-sequence-id-449549

    Enlarge

  7. V485G USP7 catalytic

    Plasmid
    #227209
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    V485G; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227209-plasmid-map-sequence-id-449548

    Enlarge

  8. P273H USP7 catalytic

    Plasmid
    #227208
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    P273H; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227208-plasmid-map-sequence-id-449547

    Enlarge

  9. Y331H USP7 catalytic

    Plasmid
    #227205
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Y331H; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227205-plasmid-map-sequence-id-449546

    Enlarge

  10. M225I USP7 catalytic

    Plasmid
    #227206
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    M225I; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227206-plasmid-map-sequence-id-449545

    Enlarge

  11. USP7 N418S catalytic

    Plasmid
    #227202
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418Sl; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227202-plasmid-map-sequence-id-449544

    Enlarge

  12. K420E USP7 catalytic

    Plasmid
    #227210
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    K420E; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227210-plasmid-map-sequence-id-449536

    Enlarge

  13. USP7 G392D catalytic

    Plasmid
    #227203
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392D; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227203-plasmid-map-sequence-id-449535

    Enlarge

  14. E345K USP7 catalytic

    Plasmid
    #227211
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    E345K; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227211-plasmid-map-sequence-id-449531

    Enlarge

  15. L373F USP7 catalytic

    Plasmid
    #227207
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L373F; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227207-plasmid-map-sequence-id-449530

    Enlarge

  16. G392A USP7 catalytic

    Plasmid
    #227204
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392A; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227204-plasmid-map-sequence-id-449529

    Enlarge

  21. pCMJ074

    Plasmid
    #215654
    Purpose
    pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143
    Depositor
    Brian Pfleger
    Article
    Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(
    Insert
    Yellow fluorescent protein
    Use
    Synthetic Biology
    Tags
    10x histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    215654-plasmid-map-sequence-id-434130

    Enlarge

Showing: 3631 - 3660 of 4144 results