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pDest-1.7kbfabp6-eGFP-pA-CrymCherry

Plasmid

#159088

Purpose

LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Inserts

1.7kb fabp6 promoter (fabp6 Zebrafish)
eGFP
polyA

Use

Fluorescent reporter

Tags

Expression

Mutation

Promoter

1.7kb fabp6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pQFDBD-2x AD*-VP16*, α-cry:EGFP

Plasmid

#180473

Purpose

Gene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mCerulean3

Plasmid

#127592

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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p9xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180479

Purpose

Inducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

9x QUAS-ZsGreen-P2A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Mouse aB crystallin 250bp promoter/AcGFP

Plasmid

#98102

Purpose

Mouse aB crystallin 250bp promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Expression

Mutation

Promoter

Alpha B-crystallin promoter 250 bp fragment (-259…

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCherry-Rab8A

Plasmid

#194333

Purpose

For use in light-induced Rab8a protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab8a (RAB8A Canis Lupus)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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p17xQUAS-Luciferase, α-cry:mCherry

Plasmid

#180482

Purpose

Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

Luciferase
17x QUAS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Mouse aB crystallin 800bp promoter/AcGFP

Plasmid

#98103

Purpose

Mouse aB crystallin 800bp promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Expression

Mutation

Promoter

Alpha B-crystallin promoter 800 bp fragment (-833…

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-PSD [N286/74R]

Plasmid

#164159

Purpose

Mammalian Expression Plasmid of anti-PSD (Zebrafish). Derived from hybridoma N286/74.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PSD (Danio rerio) recombinant mouse monoclonal antibody (dlg2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCSmChnERT(#99)

Plasmid

#184063

Purpose

cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

mCherry-nls-ERT2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180477

Purpose

Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

5x QUAS-ZsGreen-P2A
mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2plus-TDP-43

Plasmid

#162608

Purpose

Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Use

Tags

QF fusion protein

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Control-cx43.4-48H-pGTag

Plasmid

#117818

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180474

Purpose

Inducible gene expression vector pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*-EcR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

Plasmid

#162618

Purpose

Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

M337V

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP43 M337V

Plasmid

#162612

Purpose

Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

M337V

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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EIF2B3

Plasmid

#172072

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

July 18, 2021

Availability

Academic Institutions and Nonprofits only

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