Addgene

Showing: 2401 - 2420 of 12904 results

Results per page:

Sort results by:

pBOB-N-Flag-GK (12-272)

Plasmid

#134272

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 12-272

Promoter

CMV

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-N-Flag-GK_v1

Plasmid

#134270

Purpose

Lentivector encoding Flag-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 1; 524 amino acids

Promoter

CMV

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-SRS-FLAG:HIS3MX6

Plasmid

#134447

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-SRS-FLAG:kanMX6

Plasmid

#134446

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSHDY*in_PpetE:BCD2:AgBIS:Flag

Plasmid

#133971

Purpose

Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

AgBIS:Flag

Use

Synthetic Biology

Tags

Flag

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLB(N)CX-FLAG-CH3

Plasmid

#133718

Purpose

Retroviral expression vector driving CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

partial (not complete) p300 fragment, CH3 domain …

Promoter

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLB(N)CX AT-CH3-FLAG-HA WY

Plasmid

#133725

Purpose

Retroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mutation

partial (not complete) p300 fragment, AT and CH3 …

Promoter

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-C-Flag-GK5_v2

Plasmid

#134282

Purpose

Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-C-Flag-GK5_v1

Plasmid

#134281

Purpose

Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-N-Flag-GK_v2

Plasmid

#134271

Purpose

Lentivector encoding Flag-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 2; 553 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-C-Flag-GK5_v1

Plasmid

#134257

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-N-Flag-Snrnp40

Plasmid

#134249

Purpose

Lentivector encoding Flag-tagged Snrnp40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-FLAG-B-eSpCas9

Plasmid

#126772

Purpose

Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A; K1003A; R1060A; amino acids 1005-1013 repl…

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-FLAG-TAPBPR-TM-VC

Plasmid

#135499

Purpose

Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Use

Tags

Luminal/Extracellular FLAG epitope tag, Signal pe…

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Myo 9 Avi+Flag

Plasmid

#134969

Purpose

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

Depositor

Martin Bähler

Article

Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

Insert

Myo 9 (hum-7 Nematode)

Use

Tags

Avi and Flag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-ATG3 1-13 KR

Plasmid

#129299

Purpose

Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed first 13 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-ATG3 14-22 KR

Plasmid

#129300

Purpose

Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed last 9 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

Plasmid

#132683

Purpose

AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 1 (Cndp1 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge