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Showing: 6001 - 6050 of 14286 results

  2. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid
    #59313
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59313-plasmid-map-sequence-id-107095

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  3. pcDNA3-mCherry-TSC2(AA)

    Plasmid
    #192442
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192442-plasmid-map-sequence-id-379128

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  4. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187945-plasmid-map-sequence-id-373705

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  11. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Article
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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  12. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68717
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    CAG-FLEX
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68717-plasmid-map-sequence-id-120856

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  15. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68720
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    hSyn1-FLEX
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68720-plasmid-map-sequence-id-198989

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  18. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Tobias Dick
    Article
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83239-plasmid-map-sequence-id-158262

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  23. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid
    #84730
    Purpose
    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Insert
    HyPer2 targeted to nucleus
    Use
    Tags
    NLS (nuclear localisation signal and NLS (nuclear…
    Expression
    Plant
    Mutation
    Promoter
    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84730-plasmid-map-sequence-id-180307

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  24. pscAAV-Tyr

    Plasmid
    #135616
    Purpose
    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135616-plasmid-map-sequence-id-296005

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  33. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid
    #59314
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59314-plasmid-map-sequence-id-107094

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  37. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  39. pCAG_EGFP-GFE3

    Plasmid
    #79869
    Purpose
    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.
    Depositor
    Don Arnold
    Article
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Insert
    GFP-GFE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Beta-actin
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79869-plasmid-map-sequence-id-153506

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  41. MmEos3.2

    Plasmid
    #203754
    Purpose
    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Src15 n-term (SRC Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203754-plasmid-map-sequence-id-403344

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Showing: 6001 - 6050 of 14286 results