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pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(+)-Fc

Plasmid

#59314

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)

Use

Tags

Fc (from human IgG)

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p47-Strep-HA

Plasmid

#113475

Purpose

Expression of human p47 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

p47 (NSFL1C Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (1-397)

Plasmid

#42205

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-397) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-397

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HshnRNPA1_Z

Plasmid

#148112

Purpose

Mammalian Expression of HshnRNPA1

Depositor

Elisa Izaurralde

Insert

HshnRNPA1 (HNRNPA1 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation I136V compared to the tra…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIIα-Dronpa

Plasmid

#182331

Purpose

PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

RIIalpha-Dronpa (PRKAR2A Human)

Use

Tags

Full-length human PKA type 2alpha regulatory subu…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Ataxin3-Strep-HA

Plasmid

#113496

Purpose

Expression of human Ataxin-3 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Ataxin-3 (ATXN3 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Use

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ZapCY1

Plasmid

#58996

Purpose

Mitochondrial Zn-binding FRET biosensor

Depositor

Amy Palmer

Article

Park et al ACS Chem Biol. 2012 Aug 10.

Insert

mito-ZapCY1 high affinity Zn(II) sensor

Use

Tags

See comment

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-PA-mCherry

Plasmid

#138251

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-PAmCherry

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp16

Plasmid

#157724

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp16 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

A4V

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 c-SRC (91-536)

Plasmid

#42206

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (91-536) (SRC Human)

Use

Tags

Expression

Mammalian

Mutation

contains amino acids 91-536

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPE-100u

Plasmid

#13543

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-mCherry

Plasmid

#79419

Purpose

Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf1 (ARF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR(T/A)

Plasmid

#118410

Purpose

Negative-control mutant for ExRai-CKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mAIM2-Flag

Plasmid

#51537

Purpose

Express Flag-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 Tailless

Plasmid

#89466

Purpose

DSG-2 tailless control

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAKalpha_deltaCTD

Plasmid

#141196

Purpose

Expresses Strep-HA-ZAKalpha_deltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Use

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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