-
-
-
pLVX-IRES-Gαi*-BERKY3
Plasmid#158424PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsmycExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
-
pGL3-Control-PTEN-3'UTR-Mut
Plasmid#21327DepositorInsertPTEN 3' UTR Mutant (PTEN Human)
UseLuciferaseTagsExpressionMammalianMutation2 miR binding site mutated. Changed from TTTGCAC …PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
GST-N1IC
Plasmid#47612Purposebacterial expression of GST-myc-Notch1 ICDepositorInsertNotch1 intracellular domain (Notch1 Mouse)
UseTagsGST and mycExpressionBacterialMutationcontains amino acids 1753-2531PromotertacAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G07
Plasmid#67141PurposeGateway destination vector for cell-free expression with N-term myc-tag mCherry tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis-MYC and mCherryExpressionMutationPromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-E2F-3'UTR-WT
Plasmid#21170DepositorInsertE2F transcription factor 1 (E2F1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-PIG-miR-30b
Plasmid#64232PurposeExpresses miR-30b in mammalian cellsDepositorInsertmiR-30b (MIR30B Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry
Plasmid#114726PurposedCas9-VP160 expression in mammalian cellsDepositorInsertdCas9-VP160
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G08
Plasmid#67142PurposeGateway destination vector for cell-free expression with C-term mCherry myc-tag tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis-MYC and mCherryExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY1
Plasmid#158090PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY2
Plasmid#158197PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-2xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-PIG-miR-146a
Plasmid#64234PurposeExpresses miR-146a in mammalian cellsDepositorInsertmiR146a (MIR146A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-miR-20-Control
Plasmid#21169DepositorInsertmiR-20 Sensor (MIR20A Human)
UseTagsExpressionMammalianMutationSensor construct insert has two miR-20 binding si…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pMSCV-PIG-miR-26a-2
Plasmid#64230PurposeExpresses miR-26a-2 in mammalian cellsDepositorInsertmiR-26a-2 (MIR26A2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only
Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
How are we doing? Share feedback for a chance to win $50
Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more
Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more
