-
Plasmid#168207PurposeDestination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selectionDepositorTypeEmpty backboneUseUnspecified; Pdest gatewayTagsV5 APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pATD
Plasmid#141012PurposeMutisite Gateway mediated vector constructionDepositorInsertHSV-TK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DSTYK _IDG-K
Plasmid#158182PurposeGateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DSTYK (DSTYK Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-AFB2
Plasmid#83845PurposeGateway donor vector for AFB2DepositorInsertAFB2 (AFB2 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b A146T
Plasmid#83150PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationA146TPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1
Plasmid#67344PurposehSar1 with stop codon in Gateway Entry vectorDepositorInsertSAR1 (SAR1A Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a Q61L
Plasmid#83171PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationQ61LPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-mKate2-HA no-pA
Plasmid#80810Purposehemagglutinin (HA) epitope fused to C-terminus of mKate2 without pADepositorInsertmKateMyc p3E
UseTagsExpressionMammalianMutationPromoterHAAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a Q61R
Plasmid#83172PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationQ61RPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-DYRK3-V5-miniTurbo_IDG-K
Plasmid#158190PurposeGateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertDYRK3-V5-miniTurbo (DYRK3 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationR293H- please see depositor commentsPromoterUbiquitinAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12A
Plasmid#83142PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12APromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
p3E-CFP-HA no-pA
Plasmid#82591Purpose3' entry vector containing HA epitope fused to c-terminus of CFP without pA for imaging, epitope labeling/purificationDepositorInsertECFP-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CAMK1D_IDG-K
Plasmid#158167PurposeGateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMK1D (CAMK1D Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-mCherry-HA no-pA
Plasmid#82592Purpose3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purificationDepositorInsertmCherry-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L5L2
Plasmid#113738PurposeGateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only