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pBABE-puro mCherry-EGFP-LC3B

Plasmid

#22418

Purpose

retroviral expression of mCherry, EGFP-tagged LC3

Depositor

Jayanta Debnath

Article

N'Diaye et al EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.

Insert

microtubule-associated protein 1 light chain 3 beta (MAP1LC3B Human)

Use

Retroviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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CARPID BASU-dCasRx

Plasmid

#153209

Purpose

express CARPID BASU-dCasRx fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

BASU-dCasRx

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGFPGUSPlus

Plasmid

#64401

Purpose

binary plant vector expressing GFP and GUS, both driven by a 35S promoter

Depositor

Claudia Vickers

Article

Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

Inserts

GFP
GUSPlus

Use

Binary vector for plant transformation

Tags

His

Expression

Plant

Mutation

S65T

Promoter

35S

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-TagBFP2

Plasmid

#124774

Purpose

3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-IRES-EGFP

Plasmid

#64238

Purpose

Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

Depositor

Maria Lung

Article

Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTet

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

Plasmid

#193465

Purpose

Dn29 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Dn29_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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plRL117

Plasmid

#163635

Purpose

Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet-On

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Article

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Use

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +1

Plasmid

#66940

Purpose

CRISPaint frame selector +1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +1

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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