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We narrowed to 2,808 results for: sam

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AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Chemogenetics Cre-dependent expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

Plasmid

#32479

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F-GlyR-IRES-GFP

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32481

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32477

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pAEM7

Plasmid

#136422

Purpose

salL gene from S. tropica CNB-440 ligated into pHIS8

Depositor

Bradley Moore

Article

Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

Insert

salL

Use

Tags

His8 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Use

Tags

His8 tagged

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Use

Tags

His8 tagged

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Tags

Expression

Mutation

Promoter

P23

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPM_Pveg-sfgfp

Plasmid

#121503

Purpose

Integration vector that expresses gfp in S. mutans

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

Superfolder GFP

Use

S. mutans integration vector, integrates at the m…

Tags

Expression

Mutation

Promoter

Pveg

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HisSumoOphP

Plasmid

#189672

Purpose

HisSUMO tagged OphP to express OphP in pichia pastorias.

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

HisSumoOphP

Use

Tags

HisSumo tagged

Expression

Yeast

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrH

Plasmid

#225084

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Use

Tags

His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrI

Plasmid

#225085

Purpose

Expresses anthrone-oxygenase like enzyme ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrI

Use

Tags

His6 and His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-RFPKDEL

Plasmid

#138660

Purpose

Reporter plasmid expressing the ER-located RFP

Depositor

Bertrand Collet

Article

Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

Insert

RFP-KDEL

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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CROPSeq-PP7-p65-HSF

Plasmid

#196719

Purpose

CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

Depositor

Bernhard Schmierer

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

Promoter

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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EPHA1 (3KKA)

Plasmid

#25323

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

epha1.911.974 (EPHA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits