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Showing: 221 - 240 of 9005 results

  2. ECE1_101-770_HisN_AviC

    Plasmid
    #195875
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ECE1 (ECE1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195875-plasmid-map-sequence-id-387251

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  4. LIPS:SDC101a-A08:C34411

    Plasmid
    #183978
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    LIPS
    Use
    Baculovirus expresssion
    Tags
    C-terminal 8xHis tag:EFVEHHHHHHHH and N-termina…
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183978-plasmid-map-sequence-id-361418

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  5. LZRS-Mek2-K101A (KA7)

    Plasmid
    #21191
    Depositor
    Paul Khavari
    Insert
    Mek2 (MAP2K2 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    K101A (catalytically inactive)
    Promoter
    Available since
    June 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  6. phSOD1-D101G-N’His

    Plasmid
    #117704
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D101G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117704-plasmid-map-sequence-id-227520

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  7. pPD1101 Integration Test Circuit Vector

    Plasmid
    #139251
    Purpose
    Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.
    Depositor
    Joshua Leonard
    Insert
    Tus
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139251-plasmid-map-sequence-id-337940

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  8. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  9. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

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  10. pEYFP-N1-HsSmg5-del847-1017-siRNAres_I

    Plasmid
    #146540
    Purpose
    Mammalian Expression of HsSmg5-del847-1017-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del847-1017-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146540-plasmid-map-sequence-id-288986

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  11. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87407-plasmid-map-sequence-id-193449

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  12. pAAV_AR-RE_MLPmin_BC1014-luc2

    Plasmid
    #227108
    Purpose
    Androgen receptor response element for luciferase and barcode assays; barcode BC1014
    Depositor
    Michael Wehr
    Insert
    8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227108-plasmid-map-sequence-id-454379

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  13. pAAV_AR-RE_MLPmin_BC1011-luc2

    Plasmid
    #227106
    Purpose
    Androgen receptor response element for luciferase and barcode assays; barcode BC1011
    Depositor
    Michael Wehr
    Insert
    8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227106-plasmid-map-sequence-id-454369

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  14. pAAV_AR-RE_MLPmin_BC1013-luc2

    Plasmid
    #227107
    Purpose
    Androgen receptor response element for luciferase and barcode assays; barcode BC1013
    Depositor
    Michael Wehr
    Insert
    8x clustered AR response element linked to MLP minimal promoter driving barcode 1013 and a luciferase reporter gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227107-plasmid-map-sequence-id-452375

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  16. FLJ11011

    Plasmid
    #25612
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FLJ11011 (UBE2W Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. p426_Cas9_gRNA-ARS1014a

    Plasmid
    #87396
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87396-plasmid-map-sequence-id-193439

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Showing: 221 - 240 of 9005 results