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Anti-TRIP8b (exon 1a/5) [N291C/22R]

Plasmid

#114563

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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ER-P-TAG (pGS594)

Plasmid

#160039

Purpose

pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Insert

CP450TMD-APEX2-3xHA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-2xFYVE

Plasmid

#136996

Purpose

Lentiviral EGFP lipid sensor for PI(3)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-2xFYVE

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (constant) [N212/17R-2b]

Plasmid

#206664

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/17-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TRIP8b (constant) (Rattus norvegicus) recombinant Mouse monoclonal antibody (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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TRIP8b (constant) ion channel scFv [N212/17]

Plasmid

#206725

Purpose

Mammalian Expression of TRIP8b (constant) ion channel scFV. Derived from hybridoma N212/17 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TRIP8b (constant) ion channel (Rattus norvegicus) recombinant scFV (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TRIP8b (exon 1b) scFv [N212A/34]

Plasmid

#190538

Purpose

Mammalian Expression of TRIP8b (exon 1b) scFV. Derived from hybridoma N212A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TRIP8b (exon 1b) (Mus musculus) recombinant scFV (Pex5l Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (exon 1b) [N212A/34R]

Plasmid

#182108

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (exon 1b) (Mouse). Derived from hybridoma N212A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TRIP8b (exon 1b) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PH-PLC-delta-EGFP

Plasmid

#136998

Purpose

Lentiviral EGFP lipid sensor for PI(4,5)P2

Depositor

Ken-Ichi Takemaru

Insert

PH-PLC-delta-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-P4M-SidMx2

Plasmid

#136997

Purpose

Lentiviral EGFP lipid sensor for PI(4)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-P4M-SidMx2

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP-V5-AP-nes_pLX304

Plasmid

#120912

Purpose

Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

FKBP-V5-AP-NES

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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EX-HA-FRB-Cb5_pLX304

Plasmid

#120915

Purpose

Lentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

EX-HA-FRB-Cb5

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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His6x-EX-HA-FRB-MBD_pYFJ16

Plasmid

#120917

Purpose

Expresses His6-EX-HA-FRB-MBD in bacteria for protein purification

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

His6-EX-HA-FRB-MBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSJD001

Plasmid

#158589

Purpose

Pex22(1-36)-mRuby2 peroxisome marker

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

Pex22(1-36)-mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Halotag-FRB-EX-nes_pLX304

Plasmid

#120913

Purpose

Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

HA-Halotag-FRB-EX-NES

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-MCP-AP_pLX304

Plasmid

#120918

Purpose

Lentiviral vector expressing NLS-MCP-AP in mammalian nucleus

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

NLS-MCP-AP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-APEX3

Plasmid

#202590

Purpose

Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

Depositor

Reuben Harris

Article

Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

Insert

mNeonGreen-APEX3-V5 (APX1 )

Use

Retroviral

Tags

V5 epitope and mNeonGreen

Expression

Mammalian

Mutation

APEX2 L242A

Promoter

MESV LTR

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGbH500-Nanog-AXP

Plasmid

#140279

Purpose

a targeting plasmid for Nanog-APEX

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

endogenous

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBlue-loxPuro-APEX-PML_LASA

Plasmid

#140273

Purpose

a targeting plasmid for APEX-PML

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

endogenous

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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