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pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C2

Plasmid

#141247

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-SNORD115-11

Plasmid

#45890

Depositor

Stefan Stamm

Article

Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

Insert

SNORD115 without flanking exons and intron (SNORD115-11 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gRG6-SA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCREB5-donor

Plasmid

#132521

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

CREB5 (CREB5 Human)

Use

CRISPR

Tags

GFP

Expression

Mutation

none

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCre-AntiDsRed

Plasmid

#139674

Purpose

Cassette for testing Cre recombinase activity using a fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Cre recombinase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCR3.1-CREM4

Plasmid

#106459

Purpose

CMV promoter with 4 CRE sites mutated, can be used to create a reporter vector

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

4 CRE sites mutated

Use

Tags

Expression

Mammalian

Mutation

4 CRE sites mutated

Promoter

CMV

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCri-11b

Plasmid

#61333

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-B-pCR

Plasmid

#46369

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

calretinin promoter (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

promoter

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-15a

Plasmid

#61323

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-1xSunTag

Plasmid

#67177

Purpose

CRISPaint universal donor for SunTag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

1xSunTag v4

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII p16

Plasmid

#16455

Depositor

Bert Vogelstein

Article

Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

Insert

p16 (CDKN2A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pcry:GFP,-503unc:hACTA1GFP

Plasmid

#172529

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.

Depositor

Joachim Berger

Article

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

ATCA1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1822_pCR8-dCasRx

Plasmid

#118656

Purpose

Gateway Donor; dCasRx ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

No promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR01-FnCpf1

Plasmid

#176529

Purpose

Nuclease Cpf1 adapted for Aspergillus species

Depositor

Kusol Pootanakit

Article

Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

Insert

FnCpf1

Use

CRISPR; Fungal expression

Tags

Expression

Mutation

Promoter

TEF1

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCRII Pig5

Plasmid

#16499

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig5 (ANO9 Human)

Use

Tags

Expression

Bacterial

Mutation

Partial cDNA clone

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pCR8/GW:PtU6

Plasmid

#104895

Purpose

Phaeodactylum U6 promoter

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Phaeodactylum U6 promoter

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-nTEV

Plasmid

#67182

Purpose

CRISPaint universal donor for split-TEV-N fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

nTEV

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-cTEV

Plasmid

#67183

Purpose

CRISPaint universal donor for split-TEV-C fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

cTEV

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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