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Plasmid#98884PurposeA FRET sensor for Gq activation, encoded on a single plasmid.DepositorInsertmTq2-mNG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-glucose
Plasmid#225252PurposeBacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucoseDepositorInsertqmTQ2-glucose
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
Plasmid#113864PurposeSingle pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.DepositorInsertsPTCH1 (hedgehog dependent promoter)
mTurquoise2
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA_mTurquoise2
Plasmid#108237PurposeBroad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPsbA promoter - mTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM PKA Reporter
Plasmid#104551PurposeExpresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cellsDepositorInsertLyn-mTurquoise2-VASP 148 - 164
UseTagsmTurquoise 2, HA tagExpressionMammalianMutationPromoterEF1 alphaAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWKS9
Plasmid#139416PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
OMM PKA Reporter
Plasmid#104550PurposeExpresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.DepositorInsertTom20 - mTurquoise2 - VASP 148-164
UseTagsmTurqouise2ExpressionMammalianMutationPromoterEF1 alphaAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tmem98-mTq2
Plasmid#124504PurposeExpresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsmTurquoise2ExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE_mTurquoise2
Plasmid#202155PurposeLevel 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertmTurquoise2 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LAMP1
Plasmid#110948PurposeExpresses LAMP1 fused to mTurquoise2DepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-C1
Plasmid#60560PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LC3
Plasmid#110947PurposeExpresses the autophagosome marker LC3 fused to mTurquoise2DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only