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Plasmid#207091PurposepX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.DepositorInsertGGGGAGCAGATGGACCCTAC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDC1 N-terminal sgRNA
Plasmid#207090PurposepX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.DepositorInsertGTATCCTTCCCAGATCATGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT-CNP-inact
Plasmid#211804PurposeExpresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)DepositorInsert2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
UseTagsExpressionBacterialMutationH73L H153LPromotertetAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-mCardinal
Plasmid#197258PurposeExpresses the protein of mCardinal and HO1 in bacteriaDepositorInsertmCardinal
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mss4-Kina
Plasmid#202739Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmCherry:S. cerevisiae Mss4 (377-756) (MSS4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-Mss4D636K-Kina
Plasmid#202725Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-Mss4-Kina
Plasmid#202724Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mss4D636K-Kina
Plasmid#202740Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mCardinal
Plasmid#197195PurposeExpresses the protein of mCardinal in mammalian cellsDepositorInsertmCardinal
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-StefinA
Plasmid#182418PurposeBacterial expression of mNeongreen-stefinA fusionDepositorInsertmNeongreen-stefin (CSTA Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-NematocilinA
Plasmid#193010PurposePlasmid to generate an ISH probe for Hydra Nematocilin ADepositorInsertHydra Nematocilin A
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCardinal-NES
Plasmid#184453PurposeCytosolic mCardinal expression in mammalian cellsDepositorInsertmCardinal-NES
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCardinal-NLS
Plasmid#184450PurposeNucleus-targeted mCardinal expression in mammalian cellsDepositorInsertmCardinal-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCardinal-LaminB1
Plasmid#184452PurposeNuclear memebrane-targeted mCardinal expression in mammalian cellsDepositorInsertmCardinal-LaminB1
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
YSBE415: pTOP_ACA1_Vc_HOG1 terminator
Plasmid#122434PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE420:pNEO_CAC1_Vn_HOG1 terminator
Plasmid#122701PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE416: pTOP_CAC1_Vn_HOG1 terminator
Plasmid#122435PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE418:pNAT_ACA1_Vc_HOG1 terminator
Plasmid#122700PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCardinal
Plasmid#105798PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCardinalExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCardinal-TEV
Plasmid#105781PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsmCardinal-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only