Addgene

We narrowed to 834 results for: halo tag

Showing: 221 - 240 of 834 results

Results per page:

pET-51b(+)-cpHaloTag141/145

Plasmid

#194660

Purpose

Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag141/145

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag154/156

Plasmid

#194661

Purpose

Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag154/156

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagS

Plasmid

#200189

Purpose

Tom20 with reversible HaloTagS variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Use

Tags

mEGFP and reHaloTagS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagF

Plasmid

#200190

Purpose

Tom20 with reversible HaloTagF variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Use

Tags

mEGFP and reHaloTagF

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Use

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP-KDEL

Plasmid

#184904

Purpose

Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

KDEL

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-pSu9-HaloTag7-mGFP

Plasmid

#184905

Purpose

Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

Presequence of Fo-ATPase subunit 9

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJPT006-1x FLAG-HaloTag-PPF

Plasmid

#202496

Purpose

Express "PPF" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al bioRxiv 2023.04.29.538810

Insert

HaloTag

Use

Tags

1x FLAG and Palmitoylation-Palmitoylation-Farnesy…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pJPT018-LAT_WT-HaloTag-3x FLAG

Plasmid

#202502

Purpose

Express WT-LAT

Depositor

Neha Kamat

Article

Peruzzi et al bioRxiv 2023.04.29.538810

Insert

LAT (LAT Human)

Use

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pJPT019-LAT_C26A-HaloTag-3x FLAG

Plasmid

#202503

Purpose

Express C26A Lat mutant

Depositor

Neha Kamat

Article

Peruzzi et al bioRxiv 2023.04.29.538810

Insert

LAT (LAT Human)

Use

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

C26A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pJPT020-LAT_dCore-HaloTag-3x FLAG

Plasmid

#202504

Purpose

Express dCore Lat mutant

Depositor

Neha Kamat

Article

Peruzzi et al bioRxiv 2023.04.29.538810

Insert

LAT (LAT Human)

Use

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

Truncated transmembrane domain

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pJPT021-LAT_HASA-HaloTag-3x FLAG

Plasmid

#202505

Purpose

Express high ASA Lat mutant

Depositor

Neha Kamat

Article

Peruzzi et al bioRxiv 2023.04.29.538810

Insert

LAT (LAT Human)

Use

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

Several leucine subsitutions in transmembrane dom…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5 (L115R)-HaloTag

Plasmid

#205711

Purpose

Mammalian expression of DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L115R

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Tags

Expression

Mutation

S29W.A95K

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209655

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Tags

Expression

Mutation

S29W.A95K

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209656

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge