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Plasmid#153209Purposeexpress CARPID BASU-dCasRx fusion protein in mammalian cellsDepositorInsertBASU-dCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
CARPID dCasRx-BASU
Plasmid#153303Purposeexpress CARPID dCasRx-BASU fusion protein in mammalian cellsDepositorInsertdCasRx-BASU
UseCRISPR and LentiviralTagsExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU
Plasmid#179415PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASUDepositorInsertCBX7.Chromo/TAF3.PHD-13XL-BASU
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-PRPF4B_IDG-K
Plasmid#214463PurposeGateway destination clone of PRPF4B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-PRPF4B (PRPF4B Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-STK19_IDG-K
Plasmid#214465PurposeGateway destination clone of STK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-STK19 (STK19 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-RIOK1_IDG-K
Plasmid#214464PurposeGateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-RIOK1 (RIOK1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K
Plasmid#214459PurposeGateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G2 (CSNK1G2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K
Plasmid#214460PurposeGateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G3 (CSNK1G3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K
Plasmid#214458PurposeGateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G1 (CSNK1G1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK11A_IDG-K
Plasmid#214461PurposeGateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK11A (CDK11A Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - BirA*
Plasmid#131132PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntr_Myc-BirA-G3-ER
Plasmid#173814PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseGateway shuttle vectorTagsMycExpressionMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP-APP
Plasmid#196706PurposeExpresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsAP, Avitag and HAExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Myc-BirA-G3-ER
Plasmid#173815PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseTagsMycExpressionInsectMutationPromoterUASAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
rgef-1p::NmBirA
Plasmid#79971Purposeencodes E.coli biotin-ligase under neuronal promotor for C.elegansDepositorInsertBirA
UseTagsNLS::mycExpressionWormMutationPromoterrgefAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1595
Plasmid#121062PurposemKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmKate2^SEC^BioTag::AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…ExpressionWormMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1592
Plasmid#121059PurposeGFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertGFP^SEC^BioTag::AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…ExpressionWormMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1594
Plasmid#121061PurposeTagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T^SEC^BioTag::AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…ExpressionWormMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAd:CMV-rtTA-IRES-birA
Plasmid#31375DepositorInsertrtTA-IRES-BirA
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLew100_myc_BirA*
Plasmid#41716DepositorTypeEmpty backboneUseT. brucei expressionTagsExpressionMutationPromoterPARPAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only