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Addgene
Showing: 221 - 240 of 295 results
  1. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Availability
    Academic Institutions and Nonprofits only
  2. pDONR207-MUM2sp-RglA

    Plasmid
    #170720
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)
    Depositor
    Insert
    Rhamnogalacturonan lyase A
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pDONR207-MUM2sp-RglB

    Plasmid
    #170721
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)
    Depositor
    Insert
    Rhamnogalacturonan lyase B
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pDONR207-MUM2sp-PelA

    Plasmid
    #170722
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)
    Depositor
    Insert
    Pectin lyase A
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pPEE42

    Plasmid
    #139421
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.
    Depositor
    Insert
    Citrine
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
  6. pPEE43

    Plasmid
    #139422
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.
    Depositor
    Insert
    mMaroon1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
  7. pPEE45

    Plasmid
    #139424
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.
    Depositor
    Insert
    mTurquoise1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
  8. pPEE47

    Plasmid
    #139426
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.
    Depositor
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 295 results