Showing: 221 - 240 of 424 results
-
Plasmid#203662PurposeDonor plasmid for CRISPR knock-inDepositorInsertsmad1 (SMAD1 Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A TagRFP-T P2A puro
Plasmid#122200PurposeThe plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsspCas9
TagRFP-T
UseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)
Plasmid#100742PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD8A (CD8A Human)
UseTagsLOVpep and tagRFP657ExpressionMammalianMutationTruncatedPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP
Plasmid#111590PurposeAn AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromotertetOAvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP
Plasmid#86987PurposeFor cloning and expression of sgRNA together with expression of Cas9 and TagRFPDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorInsertTTF1 (NKX2-1 Human)
UseLentiviralTagsExpressionMutationPromotertetONAvailabilityAcademic Institutions and Nonprofits only -
pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]
Plasmid#159899PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | tagRFP | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mT2A-TagRFP-T-linker-dre-PBD*-FNF
Plasmid#182342PurposeRecombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNFDepositorInsertT2A-TagRFP-T-linker-dre-PBD*-FNF
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP
Plasmid#204821PurposeLentiviral vector for human SCGB3A2 promoter-driven expression of EGFPDepositorInsertAn upstream region including human SCGB3A2 promoter
UseLentiviralTagsExpressionMammalianMutationPromoterSCGB3A2 promoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G
Plasmid#204188PurposeDoxycycline inducible lentiviral vector for human NOTUM overexpressionDepositorInsertNOTUM (NOTUM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G
Plasmid#204189PurposeDoxycycline inducible lentiviral vector for human GATA6 overexpressionDepositorInsertGATA6 (GATA6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G
Plasmid#204190PurposeDoxycycline inducible lentiviral vector for human TFAP2C overexpressionDepositorInsertTFAP2C (TFAP2C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailabilityAcademic Institutions and Nonprofits only -
pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK
Plasmid#141452PurposeB6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy numberDepositorInsertCdk6
UseMouse Targeting; Dre / roxTagsC-terminal fusion of T2A-TagRFPT-dre-PBD*ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP
Plasmid#111581PurposeAn AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]
Plasmid#159898PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS
Plasmid#163083PurposeDonor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENsDepositorInsertTagRFP-T
UseTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187945PurposedCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertSpdCas9-tagRFPt-P2A-tagBFP
UseSynthetic Biology; PiggybacTagsNLS, NLS + HA-tag, P2A, tagBFP, and tagRFPtExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G
Plasmid#204191PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of R162PromoterTetONAvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
Plasmid#204210PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on R178* (*termination)PromoterTetONAvailabilityAcademic Institutions and Nonprofits only
Showing: 221 - 240 of 424 results