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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Use

Tags

Full-length LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-blast

Plasmid

#167829

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (EGFP-mRFP)

Plasmid

#18682

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (EGFP-mRFP)

Plasmid

#18680

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-blast

Plasmid

#167822

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (Cerulean-Venus)

Plasmid

#18679

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKAR

Plasmid

#60445

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (Cerulean-Venus)

Plasmid

#18681

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Mito-ExRai-AMPKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Use

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DrFLINC-AKAR-Kras

Plasmid

#182334

Purpose

Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DrFLINC-AKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-puro

Plasmid

#167821

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMDC32-Kar2-CFP-HDEL

Plasmid

#96989

Purpose

Express endoplasmic reticulum (ER) marker in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

Kar2-CFP-HDEL

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-FLEX-FLIM-AKART391A

Plasmid

#60446

Purpose

Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKART391A

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

changed Threonine 391 to Ananine

Promoter

CAG

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV x EF1a_EKAREN5(TA)-ires-puro

Plasmid

#167823

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-RAB-EKARev

Plasmid

#118477

Purpose

ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-RAB-EKARev

Use

Tags

N-terminal targeting sequence from Lyn-kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge