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Plasmid#175833PurposeExpress EGFP with plasma membrane-targeting sequence in mammalian cellsDepositorInsertEGFP with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E115 Hs.KRAS
Plasmid#70399PurposeGateway ORF clone of human KRAS [NM_004985.4] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS-A146V
Plasmid#116422PurposeLentiviral expression of KRAS A146VDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationA146VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZS041:mOrange2-KRAS
Plasmid#186432PurposemOrange2-KRASDepositorInsertmOrange2-KRAS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRKRA gRNA (BRDN0001146443)
Plasmid#76299Purpose3rd generation lentiviral gRNA plasmid targeting human PRKRADepositorInsertPRKRA (PRKRA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
(364) SB KRAB-SadCas9
Plasmid#163022PurposeSleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)DepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianMutationPromoterTET-OnAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-KRas4Bc30
Plasmid#86633PurposeExpresses mCherry-tagged membrane recruiter constructDepositorInsertFKBP:mCherry-KRas4Bc30
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.75-KRAS LCS6m
Plasmid#44571DepositorInsertKRAS 3'UTR (KRAS Human)
UseTagsExpressionMammalianMutationThe T allele at LCS6 (let-7 complementary site 6)…PromoterCMVAvailable sinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-KRasCT
Plasmid#175834PurposeExpress Venus with plasma membrane-targeting sequence in mammalian cellsDepositorInsertVenus with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D/C185S
Plasmid#83165PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D/C185S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12D/C185SPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
2_T7-mScarlet3-KRas
Plasmid#225929PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signalDepositorInsertmScarlet3-KRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrascomQ61R
Plasmid#206845PurposeTo express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationCodons altered to most optimum based on mouse cod…PromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Kraken Quartet
Plasmid#214730PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTagDepositorInsertSpyTag-Kraken Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-FLAG-KrascomQ61R
Plasmid#206863PurposeTo stably express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo.DepositorInsertKras (Kras Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationCodons 1-3 altered to most optimum based on mouse…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-FLAG-KrasnatQ61R
Plasmid#206864PurposeTo stably express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo.DepositorInsertKras (Kras Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HaloTag-KRas4B(CT)
Plasmid#214276PurposeMammalian expression of HaloTag fused to the C-terminal tail of KRas4BDepositorArticleInsertHaloTag7-KRas4B(174-188) (KRAS Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)
Plasmid#209773PurposeCRISPRi for STK4DepositorInsertdCas9-KRAB, sgSTK4i (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR)
Plasmid#209772PurposeCRISPRi for STK3DepositorInsertdCas9-KRAB, sgSTK3i (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)
Plasmid#209774PurposeCRISPRi for STK4DepositorInsertdCas9-KRAB, sgSTK4i (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only