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CoV2-Spike-D614G-N439K

Plasmid

#177970

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N439K) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, N439K

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-L452R

Plasmid

#177971

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, L452R) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, L452R

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S477N

Plasmid

#177972

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S477N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, S477N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N501Y

Plasmid

#177975

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, N501Y

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S13I

Plasmid

#177965

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S13I) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, S13I

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-T20N

Plasmid

#177966

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, T20N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, T20N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-P26S

Plasmid

#177967

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P26S) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, P26S

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-W152C

Plasmid

#177968

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, W152C)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, W152C) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, W152C

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-P681H

Plasmid

#177973

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P681H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P681H) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, P681H

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-D1118H

Plasmid

#177974

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, D1118H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, D1118H) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, D1118H

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_CoumRS

Plasmid

#64882

Purpose

Expresses Coumarinyl- tRNA synthetase

Depositor

Sebastián Brauchi

Article

Steinberg et al bioRxiv, 10.1101/065227

Insert

Coum_RS

Use

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-HA3-STONIN1

Plasmid

#177580

Purpose

Expresses Stonin1 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

STONIN1

Use

Tags

3XHA and GFP

Expression

Mammalian

Mutation

V334A- please see depositor comments

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-STONIN2

Plasmid

#177885

Purpose

Espresses Stonin2 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

stonin2 (STON2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Use

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pAJE-Ma pylRS nitroY/haloY-F5

Plasmid

#225684

Purpose

Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

Depositor

Ryan Mehl

Inserts

M. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)

Use

Methanomethylophilus alvus

Tags

Expression

Mutation

CTG125CTT, N166A, V168S, A223C, W239R

Promoter

GlnS and Lpp

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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M-PKD2 (OF2-3)

Plasmid

#21370

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

wild-type*

Promoter

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pSETT4gap

Plasmid

#153413

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Article

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

gapDH promoter from Eggerthella lenta

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-pvdA WT

Plasmid

#87098

Purpose

expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

Insert

pvdA WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSETT4tip

Plasmid

#153414

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Article

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

tipA promoter from Streptomyces lividans

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only