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  7. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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Showing: 221 - 227 of 227 results