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Plasmid#84596PurposeRenilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding siteDepositorInsertNOTCH1 3'UTR (NOTCH1 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationMutation on miR-200c binding site on NOTCH1 3…PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-LFNG 3'UTR-mir200c-mut
Plasmid#84594PurposeRenilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding siteDepositorInsertLFNG 3'UTR (LFNG Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationMutation on miR-200c binding site on LFNG 3'…PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro-KrasG12D2B
Plasmid#139786PurposeLentiviral tet-inducible expression of KrasG12D (mouse)DepositorInsertKrasG12D (Kras Mouse)
UseLentiviralTagsExpressionMammalianMutationG12DPromoterAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3026
Plasmid#144502PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREB1 (CREB1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2364
Plasmid#142051PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREB1 (CREB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG059 MBP-CREB-His
Plasmid#154076PurposeExpresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120 mut1&2
Plasmid#35546DepositorUseLuciferaseTagsluciferaseExpressionMutationT to G at position -52 and T to G at position -96…PromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120 Ebox mut1
Plasmid#35544DepositorUseLuciferaseTagsluciferaseExpressionMutationT to G at position -96 in promoterPromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120 Ebox mut2
Plasmid#35545DepositorUseLuciferaseTagsluciferaseExpressionMutationT to G at position -52 in promoterPromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF3400
Plasmid#144876PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGMEB1 (GMEB1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1290
Plasmid#143782PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGMEB1 (GMEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0365
Plasmid#142605PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCEB1 (ELOC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti 4.1 Ex miR200b-200a-429
Plasmid#35533Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF086 MBP-CREB 127-135-His
Plasmid#154075PurposeExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-630)
Plasmid#171086PurposeCo-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorUseTagsHisExpressionBacterialMutationDeleted N-term 26aa and C-term 142aa for mutant e…PromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(400-772)
Plasmid#171087PurposeCo-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorUseTagsHisExpressionBacterialMutationDeleted N-term 26+399 amino acids for mutant expr…PromoterT5 promoter and T7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-772)
Plasmid#171085PurposeCo-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorUseTagsHisExpressionBacterialMutationDeleted N-term 26 amino acids for expression purp…PromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L121A A129M F153L WT*
Plasmid#18550DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationL121A A129M F153L WT*PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L121M L133V F153L WT*
Plasmid#18557DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationL121M L133V F153L WT*PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L121A A129V L133A F153L WT*
Plasmid#18551DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationL121A A129V L133A F153L WT*PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only