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Showing: 221 - 240 of 420 results

  3. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Mark Sellmyer
    Article
    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Expression
    Mutation
    None
    Promoter
    EF1a
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    193253-plasmid-map-sequence-id-383064

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  7. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Use
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

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  11. pSL24a-Linker-smHA-FP-DHFR-LoxP

    Plasmid
    #166238
    Purpose
    Tagging of weakly expressed genes, floxed DHFR marker
    Depositor
    David Sibley
    Insert
    Spaghetti monster HA
    Use
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166238-plasmid-map-sequence-id-329831

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  12. pSL24b-Linker-smMyc-FP-DHFR-LoxP

    Plasmid
    #166239
    Purpose
    Tagging of weakly expressed genes, floxed DHFR marker
    Depositor
    David Sibley
    Insert
    Spaghetti monster Myc
    Use
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166239-plasmid-map-sequence-id-333396

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  20. pLenti CMV Puro DHFR.dn-cHSF1

    Plasmid
    #134729
    Purpose
    Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.
    Insert
    DHFR.dn-cHSF1 (HSF1 Human)
    Use
    Lentiviral
    Tags
    DHFR
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 186-202, 379−529
    Promoter
    CMV
    Available since
    Aug. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    134729-plasmid-map-sequence-id-271703

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Showing: 221 - 240 of 420 results