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5'-BirA-FLAG Kdm1a-exon minimal CMV pCDNA5

Plasmid

#212088

Purpose

LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a-exon (Kdm1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Kdm1a+exon minimal CMV pCDNA5

Plasmid

#212089

Purpose

LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a+exon(12nt) (Kdm1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5

Plasmid

#212091

Purpose

LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt) (Chtop Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Sap30bp-exon minimal CMV pCDNA5

Plasmid

#212092

Purpose

LR vector for integration of Sap30bp-exon into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp-exon (Sap30bp Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Sap30bp+exon minimal CMV pCDNA5

Plasmid

#212093

Purpose

LR vector for integration of Sap30bp+exon(48nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp+exon(48nt) (Sap30bp Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

Plasmid

#184645

Purpose

Retroviral expression of BirA Rap1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1-I312R

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

Plasmid

#138417

Purpose

CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Inserts

BirA
dCas9

Use

Lentiviral

Tags

FLAG, Avi-tag and V5, His

Expression

Mutation

Promoter

EF1a

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

Plasmid

#120565

Purpose

Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

Plasmid

#120567

Purpose

Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

Plasmid

#120566

Purpose

Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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COC053

Plasmid

#209131

Purpose

Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

Depositor

Christopher A. O'Callaghan

Article

O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

Insert

Enzyme BirA (birA E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTJK421

Plasmid

#138007

Purpose

BirA*-SIX2 expression. Vector also expresses GFP under control of hUBC promoter

Depositor

Tami Kingsbury

Insert

BirA* tagged SIX2 (SIX2 Human)

Use

Lentiviral

Tags

BirA*

Expression

Mutation

None

Promoter

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1518

Plasmid

#62388

Purpose

pFA6a -mCherry-BirA::HIS5

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

mCherry-BirA

Use

Yeast c-terminal tagging vector

Tags

mCherry-BirA

Expression

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTJK368

Plasmid

#138006

Purpose

BirA* expression. Vector also expresses GFP under control of hUBC promoter

Depositor

Tami Kingsbury

Insert

BirA*

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1507

Plasmid

#62361

Purpose

pFA6a -mVenus-BirA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

c-terminal mVenus-BirA tagging

Use

Yeast c-terminal tagging vector

Tags

mVenus-BirA

Expression

Mutation

L232H mutation compared to standard mVenus

Promoter

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1508

Plasmid

#62362

Purpose

pFA6a BirA-mVenus-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ssh1

Use

Tags

BirA-mVenus

Expression

Yeast

Mutation

Promoter

Gal1

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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RENBP_11-427_BioN

Plasmid

#195869

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RENBP (RENBP Human)

Use

Pfbd-bira, baculovirus expression

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Mutation

Promoter

Polyhedrin

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDP085

Plasmid

#198425

Purpose

pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

Depositor

Markus Schmid

Article

You et al Nat Commun. 2017 May 17;8:15120. doi: 10

Insert

E. coli BirA

Use

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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