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pRAC01A
Plasmid#193730PurposeExpresses deactivated-Cas9 (J23101) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAC07A
Plasmid#193733PurposeExpresses deactivated-Cas9 (J23107) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAC10A
Plasmid#193734PurposeExpresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertionDepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5F-ALK5-HA(ecto)
Plasmid#31720DepositorInsertALK5 (TGFBR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTH278-GST-TEV-SUP45
Plasmid#29743DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsGSTExpressionBacterialMutationwild typePromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 TBRI K to R
Plasmid#19159DepositorInserttransforming growth factor, beta 1 (TGFBR1 Human)
UseTagsExpressionMammalianMutationK420R mutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTH340-SUP45(1-242)-GalBD
Plasmid#29740DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 1-242 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJJB1535
Plasmid#218613PurposeExpress Pex5DepositorInsertPex5
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1536
Plasmid#218617PurposeExpress Pex5 and Pex11DepositorInsertPex5
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1376
Plasmid#218618PurposeExpress Pex5, Pex3, Pex19, and Pex8DepositorInsertPex5
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1577
Plasmid#218619PurposeExpress Pex5, Pex2, Pex17, and Pex10DepositorInsertPex5
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINER3R
Plasmid#185883PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER4R
Plasmid#185884PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH339-SUP45(1-138)-GalBD
Plasmid#29739DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 1-138 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAKlux2
Plasmid#14080DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-NT
Plasmid#37818DepositorInsertPromotorless gfp reporter gene
UseTagsExpressionMutationPromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only
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