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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold

Plasmid

#186981

Purpose

mammalian expression of Cas7-11 crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

mature DR guide

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain

Plasmid

#183396

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

Plasmid

#172508

Purpose

Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Plasmid

#172514

Purpose

Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64T X11 (DM#103)

Plasmid

#15043

Depositor

Douglas Melton

Article

Ku et al Development. 1993 Dec . 119(4):1161-73.

Insert

Xwnt-11 (wnt11b.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pAc5.1C-FLuc-V5His6

Plasmid

#21183

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

Firefly Luciferase

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pPS2169

Plasmid

#1364

Depositor

Pamela Silver

Article

Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

Insert

RPL11B (RPL11B Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pPS2167

Plasmid

#1365

Depositor

Pamela Silver

Article

Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

Insert

RPL11B (RPL11B Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pPS2168

Plasmid

#1366

Depositor

Pamela Silver

Article

Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

Insert

RPL11B (RPL11B Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

5BoxB

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPT11 sh1 shRNA

Plasmid

#122020

Purpose

Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPT11 sh2 shRNA

Plasmid

#122023

Purpose

Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Use

Tags

his tag on Spc34 and strep tag on Dad1

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP-Hybrid-caspase_mouse-caspase-11-CARD+canine-Caspase-1/4-catalytic domain

Plasmid

#183398

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of mouse caspase-11 fused to catalytic domain of canine caspase-1/4

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo.mCherry-NEDD4

Plasmid

#38316

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

Nedd4 (NEDD4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1A-Dm-bantam

Plasmid

#22788

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

bantam (ban primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-TREX1

Plasmid

#27218

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

n/a

Promoter

Availability

Academic Institutions and Nonprofits only