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pDSG375

Plasmid

#115603

Purpose

surface-displayed Nb2; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Bxb1-His

Plasmid

#123132

Purpose

Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Bxb1 integrase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Plasmid

#207860

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Use

AAV

Tags

Expression

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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psPax2-D64V-NC-mNeon

Plasmid

#196509

Purpose

mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

mNeon

Use

Tags

mNeon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 3

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Expression

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-iCT

Plasmid

#129094

Purpose

KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139089

Purpose

Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

xCas9-NG

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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