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Plasmid#225149PurposeGateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editingDepositorInsertLoA3GX1-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJB67
Plasmid#226316PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with K to A CsoS2 MR1-6
UseTagsExpressionBacterialMutationK261A, K320A, K380A, K436A, K495A, K546APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB68
Plasmid#226315PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-7
UseTagsExpressionBacterialMutationY300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB74
Plasmid#226314PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-5
UseTagsExpressionBacterialMutationY300A, Y359A, Y420A, Y475A, Y534APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB73
Plasmid#226313PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-3
UseTagsExpressionBacterialMutationY300A, Y359A, Y420APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB51
Plasmid#226308PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1
UseTagsExpressionBacterialMutationVTG273-275AAA, VTG284-286AAA, VTG295-297AAAPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB48
Plasmid#226307PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-6
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369S, C467S, C485S, C526S, …PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB47
Plasmid#226306PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-5
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369S, C467S, C485S, C526S, …PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB46
Plasmid#226305PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-4
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369S, C467S, C485SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB45
Plasmid#226304PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-2
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM2
Plasmid#225289PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC311 for pJBM2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM3
Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)
Plasmid#218640PurposeGateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3 R262W (disease-associated mutation)
Plasmid#218647PurposeGateway shuttling vector for cloning NLRP3-R262W (no stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsExpressionMutationR262WPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3(ΔPYD, I125M-END)
Plasmid#218641PurposeGateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMGA
Plasmid#139931PurposeM. magneticum/E. coli shuttle vector (pMGT and p15A ori)DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23119Available sinceMarch 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_HD_G1333-DddA11'-C
Plasmid#199797PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1333-DddA11'-C
Plasmid#199798PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddA11'-C
Plasmid#199799PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only