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pcDNA3-Flag-Cortactin

Plasmid

#74476

Purpose

This plasmid was constructed to study cortactin interactions

Depositor

J Thomas Parsons

Article

Weed et al J Cell Biol. 2000 Oct 2;151(1):29-40.

Insert

Cortactin (Cttn Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdc2 (716)

Plasmid

#61840

Purpose

mammalian expression of cdc2

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

cdc2 (CDK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
IFNAR2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176938

Purpose

Mammalian expression vector encoding IFNAR2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

IFNAR2 (IFNAR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CRT-GFP pcDNA3.1

Plasmid

#80978

Purpose

mammalian expression of CRT-GFP

Depositor

Peter Steyger

Article

Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

Insert

CRT (Calr Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

no KDEL

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

Plasmid

#141198

Purpose

Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Use

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids and amino acids 6…

Promoter

CMV

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB8

Plasmid

#19531

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA,3Q)

Plasmid

#192444

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_STf

Plasmid

#182006

Purpose

CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

LAMP1_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Kohinoor 2.0/pcDNA3

Plasmid

#182315

Purpose

Positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

Insert

Kohinoor 2.0

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB9

Plasmid

#19532

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 Sense

Plasmid

#60631

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1delta

Plasmid

#124142

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mPax3-3xHA

Plasmid

#27319

Depositor

Jonathan Epstein

Article

Epstein et al J Biol Chem. 1995 May 19. 270(20):11719-

Insert

Pax3 (Pax3 Mouse)

Use

Tags

3X HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3/mArf3(T31N)-HA

Plasmid

#79416

Purpose

Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf3(Q71L)-HA

Plasmid

#79415

Purpose

Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cdt1 human

Plasmid

#72681

Purpose

mammalian expression of human Cdt1

Depositor

Domenico Maiorano

Article

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdt1 (CDT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS(KaganF57)

Plasmid

#52000

Purpose

Expresses the human MAVS CDS containing "weak translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pY128 (pcDNA3.1-U6-huMb2Cpf1)

Plasmid

#92304

Purpose

Expression of humanized Mb2Cpf1 and crRNA

Depositor

Feng Zhang

Insert

huMb2Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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