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pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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V405 4HA-CKIe

Plasmid

#13724

Depositor

David Virshup

Article

Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

Insert

casein kinase 1 epsilon (CSNK1E Human)

Use

Tags

4 HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Zeo-CnA-H151Q

Plasmid

#191967

Purpose

Expression of dominant negative CnA H151Q mutant protein (catalytically inactive).

Depositor

Luca Scorrano

Article

Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

Insert

Calcineurin subunit alpha H151Q (PPP3CA Human)

Use

Tags

Expression

Mammalian

Mutation

H151Q

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 WT

Plasmid

#78260

Purpose

psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

fully complimentary let-7 target site

Use

Luciferase; Microrna activity

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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phage UBC NLS-HA-2XMCP-tagRFPt

Plasmid

#64541

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, NLS, and tagRFP-T

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

Plasmid

#166610

Purpose

Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

ChR2-TdTomato

Use

AAV

Tags

Expression

Mutation

H134R

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLCa

Plasmid

#196912

Purpose

Visualization of clathrin-coated pit formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

Insert

Clathrin-Coated light chain

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT4_HA

Plasmid

#24482

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Sirtuin 4 (SIRT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-WT

Plasmid

#210722

Purpose

Construct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9

Plasmid

#28100

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-wild type

Plasmid

#61003

Purpose

Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 (PTPN14 Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
DsRed-Rab5 WT

Plasmid

#13050

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Use

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); serine …

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab7

Plasmid

#174458

Purpose

Express mCherry with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with late endosome-targeting sequence

Use

Tags

small GTPase (Rab7)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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