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Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
UseTags3x mycExpressionYeastMutationPromoterADH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)
Plasmid#166609PurposeEncodes Cre-inactivated hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)
Plasmid#166611PurposeEncodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TREDepositorInsertEGFP-2A-Tetanus Toxin
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
myc-mTOR (1271-2008 aa)
Plasmid#21746DepositorInsertmTOR (1271-2008) (Mtor Rat)
UseTagsmycExpressionMammalianMutationamino acids 1271-2008PromoterAvailable sinceAug. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2A
Plasmid#24180PurposeMammalian expression of human JMJD2A with HA tagDepositorInsertJMJD2A (KDM4A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-4EBP1_4xAla
Plasmid#38240DepositorInsert4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationT37A, T46A, S65A, T70A (dominant negative)PromoterTet ONAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor
Plasmid#1860PurposePlease see Addgene item #11367 for an updated version of this plasmid.DepositorInsertRictor (RICTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pCDH_Str-KDEL_ManII-SBP-EGFP
Plasmid#65258Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1 (1-599)
Plasmid#127461PurposeExpressing Flag-tagged human MCPIP1 full lengh protein in mammalian cellsDepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9
Plasmid#84247PurposeExpresses direct fusion VPR-Sp dCas9DepositorInsertsVPR-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, VPR, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLCa
Plasmid#196912PurposeVisualization of clathrin-coated pit formation in living mammalian cellsDepositorInsertClathrin-Coated light chain
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-TagRFP
Plasmid#162035PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsRFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-mCherry
Plasmid#65253Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b KD
Plasmid#16262DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationKinase dead: K85APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1hSIRT5_HA
Plasmid#24483DepositorInsertSirtuin 5 (SIRT5 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-4JC1RR
Plasmid#190847PurposeFor mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-NL1F50
Plasmid#190831PurposeFor mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA6-IgK-hFRa(23-234)C11S-th-Fc
Plasmid#177841PurposeMammalian Expression of Folate ReceptorDepositorInsertFolate receptor alpha (FOLR1 Human)
UseTagsExpressionMammalianMutationall 11 C mutated to SPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-renilla
Plasmid#38235DepositorInsertEef2 5'UTR / Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationEef2 5' UTR fused to renilla ORFPromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only