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Plasmid#173997PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationR33A in rAPOBEC1 and D10A in zCas9PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2E
Plasmid#185866PurposeKnocking out ALD6DepositorInsertALD(-125, 40)- ALD6(1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-DEN1
Plasmid#183487PurposeTo express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.DepositorInsertClover4-LUMABS-DEN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAID5WY
Plasmid#165050PurposeIntegrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH42(CUG/A)
Plasmid#154132PurposeExpresses engineered (23S-5S phusion) rRNAs operon in bacterial cellsDepositorInsertengineered bacterial rRNAs operon under control of pL promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D977A
Plasmid#129674PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD977A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-E848A
Plasmid#129675PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsE848A; AacCas12b is rice codon optimizedExpressionPlantMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A
Plasmid#129676PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD573A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D951A
Plasmid#129677PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD951A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-E827A
Plasmid#129678PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationE827A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D977A
Plasmid#129680PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationD977A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-E848A
Plasmid#129681PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationE848A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK224
Plasmid#110590PurposeBTK Type 3 coding sequence for golden gate assemblyDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa1.2
Plasmid#136373PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.3
Plasmid#136374PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.3
Plasmid#136377PurposeGateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa1.2.3
Plasmid#136372PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP
Plasmid#99730Purposeoverexpression of Tmeff2 and mCherryDepositorInsertTmeff2 (Tmeff2 Mouse)
UseTags2xMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP
Plasmid#99729Purposeoverexpression of Tmeff2 and CopGFPDepositorInsertTmeff2 (Tmeff2 Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only