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Plasmid#112129Purposeempty sgRNA cloning vector with MS2-AID3fDepositorInsertAIDmono 3f mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL2 sgRNA(MS2)-MS2-AID3c
Plasmid#112128Purposeempty sgRNA cloning vector with MS2-AID3cDepositorInsertAID mono 3c mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-LacZ-GFP-Puro (BB)
Plasmid#170459PurposeFor cloning gRNA blockDepositorInserthU6-BB-LacZ and EF1A-EGFP-2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 for gRNA and EF1A for EGFP-2A-PuroAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1
Plasmid#192187PurposeTdgA vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast
Plasmid#71216PurposedCas9 fused to human DNMT3A catalytic domainDepositorInsertdSpCas9-DNMT3A catalytic domain
UseLentiviralTagsdSpCas9-DNMT3AExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AmCyan
Plasmid#138481PurposeFor cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.DepositorInsertAmCyan
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A
Plasmid#66819PurposedCas9 fused to BFP and the human DNMT3A catalytic domainDepositorInsertdSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
UseTagsdSpCas9-BFP-DNMT3AExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast
Plasmid#71218PurposedCas9 fused to the human DNMT3A catalytically inactive domainDepositorInsertdSpCas9-DNMT3A(E752A)
UseLentiviralTagsSPdCas9-DNMT3A(E752A)ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3B
Plasmid#66820PurposedCas9 fused to BFP and the human DNMT3B catalytic domainDepositorInsertdSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
UseTagsdSpCas9-BFP-DNMT3BExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJJR50
Plasmid#75026PurposeU6 promoter driven flipped + extended sgRNA expression vectorDepositorInsertguide RNA, flipped and extended version
UseCRISPRTagsExpressionMutationPromoterR07E5.16 (U6)Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pROF441
Plasmid#155336PurposeBinary vector for expression of a gRNA targeting AtAP1 promoterÂDepositorInsertLB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARB367
Plasmid#124050PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-BFP
Plasmid#164273PurposeEmpty sgRNA expression vector with co-expression of BFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EFSAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 KanR foundation lox casette
Plasmid#132388PurposeFoundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locusDepositorInsertG418 resistance
UseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterpromoterlessAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterMaize ubiquitin 1 (ZmUbi1)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-mScarlet-I-CD4-bla
Plasmid#189980PurposeDonor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GLS-3xFLAG
Plasmid#163450Purposelentiviral overexpression vector for human GLSDepositorInsertGLS (GLS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-mEGFP-Actin
Plasmid#119870PurposeAAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta ActinDepositorInsertbeta Actin (Actb Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseCRISPRTagsExpressionMutationPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-mEGFP-camk2a
Plasmid#104589PurposeAAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2aDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterNoneAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only