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NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75233

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.1

Plasmid

#81049

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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BRCA1-(170-360)

Plasmid

#35066

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 170-360

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 ATG m7

Plasmid

#52035

Purpose

encodes mutant HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

all seven let-7 sites in the 3′ UTR mutated (‘m7’)

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pCA528 huPQBP1

Plasmid

#217449

Purpose

Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA E45A

Plasmid

#217438

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Tags

Expression

Mutation

CA E45A

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA M66I

Plasmid

#217440

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Tags

Expression

Mutation

CA M66I

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA Q67H/N74D

Plasmid

#217442

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Tags

Expression

Mutation

CA Q67H,N74D

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pProEx Rac1(G12C, K96H) CL

Plasmid

#224270

Purpose

Protein purification of Rac1 (G12C, K96H)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Bacterial

Mutation

G12C, K96H

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-marmoset cGASdeltaN-HA

Plasmid

#186863

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag

Use

Retroviral

Tags

HA

Expression

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-WHG cGASdeltaN-HA

Plasmid

#186868

Purpose

Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

White-handed gibbon cGAS truncation mutant (193-557 a.a.) with C-terminal HA tag

Use

Retroviral

Tags

HA

Expression

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsRUNX

Plasmid

#205639

Purpose

reRNA guide targeting Homo sapiens RUNX

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsRUNX (RUNX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmPCSK9

Plasmid

#205634

Purpose

reRNA guide targeting Mus Musculus PCSK9

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmPCSK9 (Pcsk9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S)

Plasmid

#224282

Purpose

Cell transfection and expression of Rac1 (G12C, K96H, P29S)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12C, K96H, P29S

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

Plasmid

#224281

Purpose

Cell transfection and expression of Rac1 (G12C, K96H)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12C, K96H

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)

Plasmid

#224277

Purpose

Cell transfection and expression of Rap1A (G12C, L96F)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Use

Tags

Expression

Mammalian

Mutation

G12C, L96F

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SOX17

Plasmid

#222925

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOX17 (SOX17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA233

Plasmid

#215941

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA315

Plasmid

#215951

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA232

Plasmid

#215940

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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