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Plasmid#196890PurposeNeuron-specific expression of human (h)Centrin fused to mNeonGreenDepositorInsertmNeonGreen-hCentrin1 (CETN1 Human)
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE
Plasmid#168106PurposePiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloningDepositorInsertmCherry-KASH
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterCAGAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA
Plasmid#216321PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice acceptor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216249PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminusDepositorInsertCas9 and sgRANGAP.C1 (RANGAP1 Human)
UseCRISPR; Endogenous taggingTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180156PurposetdTomato cre reporter with Hipp11 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
Plasmid#108833PurposeExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDsDepositorInsertFlp recombinase fragment aa28 to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLS and GAIExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180153Purposetd-sfGFP dre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#68719PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6f
UseAAVTagsExpressionMutationPromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#68717PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationCre-dependent expression from inverted open readi…PromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
Plasmid#114078PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons
Plasmid#172479PurposetdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin geneDepositorInserttdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR
Plasmid#171965PurposeDonor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cellsDepositorInsertSynthetic CLTX CD32a ITAM CD3z CAR
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine
Plasmid#104122PurposesynOptopatch constructDepositorInsertspine-jRGECO1a-P2A-QuasAr-dark Citrine
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS
Plasmid#206115Purposeexpression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320A, E670A, E1411A, E1707APromoterCMV/B-actinAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216251PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminusDepositorInsertCas9 and sgSEC61B.N1 (SEC61B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)
Plasmid#114094PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-RR (S542R/K607R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationS542R and K607RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast
Plasmid#213826PurposeExpresses human PRDM6 with a C-terminal V5 epitope tag.DepositorInsertPRDM6 (PRDM6 Human)
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA
Plasmid#196896PurposeNeuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmidsDepositorInsertN-Cadherin-2xHA (Cdh1 Mouse)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP
Plasmid#180151Purposetd-sfGFP cre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry
Plasmid#114726PurposedCas9-VP160 expression in mammalian cellsDepositorInsertdCas9-VP160
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only