-
Plasmid#80603PurposeExpresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus endDepositorInsertEB1 (MAPRE1 Human)
UseTagsmCherryExpressionMammalianMutationTruncated C-terminus; EB1 coding sequence ends at…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
UseTagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha
Plasmid#58256Purposebacterial expression of human PITP alphaDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Pou4f3
Plasmid#158530PurposeExpresses human Pou4f3 in mammalian cellsDepositorInsertPOU Class 4 Homeobox 3 (POU4F3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DCX-EGFP
Plasmid#80599PurposepLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)DepositorInsertDoublecortin (DCX Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_empty
Plasmid#203811PurposePlasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti F9-TetR-EGFP-IRES-PuroR
Plasmid#117049Purposelentiviral vector for expression of TetR-EGFP, for visualization of TetO repeatsDepositorInsertF9-TetR-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterF9 promoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo1-G3BP1_pTRE-G418
Plasmid#202015Purposeexpresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-G3BP1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-1
Plasmid#208790PurposeInducibly expresses human Caspase1, fused to light-activatable Cry2DepositorInsertCaspase1 (CASP1 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS614_pTetR-P2A-BFPnls/sgNS
Plasmid#108650PurposeNon-specific Spy sgRNA under U6 promoterDepositorInsertsNon-specific sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mBlueberry2-P2A-EGFP
Plasmid#184937PurposeExpresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cellsDepositorInsertmBlueberry2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…TagsExpressionPlantMutationPromoterE12-omegaAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
LZRS-hNEDD9_IresGFP
Plasmid#21962DepositorInsertNEDD9 (NEDD9 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 wt (Nigg RG6)
Plasmid#41160DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hTel2
Plasmid#30214DepositorInserttelomere maintenance 2 (TELO2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-PSD95.FingR-eGFP-CCR5TC
Plasmid#125691PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE2-luc2P-Hygro
Plasmid#101790PurposeLuciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)DepositorInsert3xERSE-II
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra1-P2A-EGFP
Plasmid#184935PurposeExpresses blue fluorescent protein Electra1 and EGFP in mammalian cellsDepositorInsertElectra1-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bmi1-overexpression
Plasmid#21577DepositorInsertBmi-1 (Bmi1 Mouse)
UseLentiviralTagsires-eGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-dsRed-shscramble
Plasmid#71384PurposeExpresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cellsDepositorInsertCMV promoter-dsRed-mir30-shscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterCMV enhancer/promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta WT GFP
Plasmid#128820PurposeTo visualize Doc2beta WT in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-humanTRIM5-FOS
Plasmid#79066PurposeExpress human TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(human)-FOS (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pflareA-psd95-exon18.
Plasmid#90249PurposeMinigene reporter for alternative splicing analysisDepositorInsertpsd95-exon18 (Dlg4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase
Plasmid#163404PurposeExpresses BMPR-2 lacking the kinase domain in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pflareG-psd95-exon18
Plasmid#90266PurposeMinigene reporter for alternative splicing analysisDepositorInsertPsd95-exon18 (Dlg4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pk7-GR-GFP
Plasmid#15534DepositorInsertGlucocorticoid receptor (NR3C1 Human)
UseTagsGFPExpressionMammalianMutationThe glucocorticoid receptor was cloned into the X…PromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-Q76L (CA)
Plasmid#83422PurposeMouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 76 from Q to L (Q76L: CAG -> CTG)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Brm WT-Flag pShuttle
Plasmid#122105PurposeExpression of full length Brm in mammalian cellsDepositorInsertBRM (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GPI
Plasmid#203759PurposeEncodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.DepositorInsertCD58 c-term (CD58 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-T31N (DN)
Plasmid#83423PurposeMouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 31 from T to N (T31N: ACG -> AAC)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag-RARalpha
Plasmid#35555DepositorInsertRARalpha (RARA Human)
UseTagsFlag and HisExpressionMammalianMutationPromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSP2-48-merTetO-EFS-BLaR
Plasmid#118712PurposeContains an optimized 48-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
UseTagsExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG mEmerald-LAMP1
Plasmid#168513PurposeExpression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomesDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmEmeraldExpressionMammalianMutationPromoterCAGAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-PE2-His
Plasmid#201973PurposeExpress His-tagged PE2 in bacteriaDepositorInsertPE2-HIS
UseTagsHis and c-myc NLSExpressionBacterialMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro hTERT shRNA
Plasmid#10688DepositorInserthTERT shRNA (TERT Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-hM4Di-mCherry
Plasmid#80947Purposehuman AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoterDepositorInserthM4Di-mCherry
UseTagsmcherry fusionExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-hM3Dq-mCherry
Plasmid#80948Purposehuman AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoterDepositorInserthM3Dq-mCherry
UseTagsmcherry fusionExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS613_pTetR-P2A-BFPnls/sgTelo
Plasmid#108649PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInsertsTelomere-targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FP
Plasmid#203757PurposeEncodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertNRas c-term (NRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only