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Plasmid#205186PurposeThis vector encodes of a synCAM (iCAM1) and GFPDepositorInsertsynCAM iCAM1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Egfr(Velvet)
Plasmid#18788DepositorInsertepidermal growth factor receptor (Egfr Mouse)
UseTagsFLAGExpressionMammalianMutationD833G; signal sequence removed (aa1-24)PromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP71-IFN-B
Plasmid#174597PurposeExpresses secreted murine interferon betaDepositorInsertsecreted IFN-beta
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-APEX3
Plasmid#202590PurposeGamma-retroviral vector that expresses mNeonGreen fused to APEX3.DepositorInsertmNeonGreen-APEX3-V5 (APX1 )
UseRetroviralTagsV5 epitope and mNeonGreenExpressionMammalianMutationAPEX2 L242APromoterMESV LTRAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6sheLLr
Plasmid#37318DepositorInsertHPV6L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME.E (pDHg16)
Plasmid#24573DepositorInsertpME.E (pDHg16)
UseTagsExpressionMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi
Plasmid#138106PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFerritinH
Plasmid#122652PurposeExpression of hFerritinH in bacterial cellsDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK289 (mAID-mClover-NeoR)
Plasmid#72827PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Neo
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP1-10
Plasmid#78591PurposeExpress mCherry-GFP1-10 in mammalian cellsDepositorInsertGFP 1-10
UseTagsmCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-Sec61β
Plasmid#172442PurposeExpression of ER protein Sec61beta with an N-terminal FKBP-GFP tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsFKBP-GFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tom20-V5-FKBP-AP_pLX304
Plasmid#120914PurposeLentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membraneDepositorInsertTom20-V5-FKBP-AP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL1-FLAG
Plasmid#15818DepositorInsertCullin1 (CUL1 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N452PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTOX11
Plasmid#127456PurposeEncodes MqsR toxin. Gent-RDepositorInsertmqsR toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-CUL5
Plasmid#19895DepositorInsertcullin 5 (CUL5 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
UseTagsHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorUseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-RPS27A-UTR-PP7-WPRE
Plasmid#198339PurposePB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loopsDepositorInsertRPS27A-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loopsExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 PuroR
Plasmid#84386PurposeLentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3K(1-10)
Plasmid#157993PurposeLentiviral vector of constitutive expression of mNeonGreen3K(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationG27D, D42E, M128I, K153M, T170IPromoterSFFVAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTOX10
Plasmid#127455PurposeEncodes YhaV toxin. Gent-RDepositorInsertyhaV toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVM_02
Plasmid#204500PurposeExpression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)DepositorInsertamino acids 54-213 VHL (VHL Human)
UseTags6xHis, TEV, and ThrombinExpressionBacterialMutation0PromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS654 All-in-One AAV-sgRNA-hNmeCas9
Plasmid#112139PurposeDelivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.DepositorInsertssgRNA scaffold
Human-codon-optimized NmeCas9
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterU1a and U6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA
Plasmid#178534PurposeModifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-FUW-eGFP-RHOA-Q63L
Plasmid#73081PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63LDepositorInsertRHOA-Q63L (RHOA Human)
UseLentiviralTagseGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AlaRS
Plasmid#124103PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAlanyl-tRNA ligase (alaS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWP78
Plasmid#61263PurposeSmall IncP-based broad host range replicating vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
opto-Tiam1
Plasmid#174508PurposeExpresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-Tiam1-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_526
Plasmid#184859PurposeLentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
UseTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actin promoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PIP2-GFP(ST) sensor
Plasmid#170356PurposeGFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.DepositorInsertGFP(ST)-PH (PLCD1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWS5397-CRISPRai-NatR
Plasmid#182836PurposeSaccharomyces cerevisiae inducible CRISPRai vector - NatR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSVBneoT
Plasmid#22883DepositorInsertEarly region SV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
460 p3E-2A-mCherrypA
Plasmid#195970PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCherry
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CW3SL-EGFP
Plasmid#61463PurposeAAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Cre
Plasmid#170367PurposeAAV vector expressing Cre under human synapsin promotorDepositorInsertCre
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE16
Plasmid#90357PurposeCREB - Cyclic AMP gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCREBAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-wt-pBabe-puro
Plasmid#19365DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt human cDNAPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest Neo
Plasmid#62178PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF0474 Ef1a-Si74_attB acceptor
Plasmid#193475PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertSi74_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only