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Showing: 2361 - 2380 of 3119 results

  1. pMTB2-Avi-Cerulean-RanGAP

    Plasmid
    #79878
    Purpose
    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79878-plasmid-map-sequence-id-149739

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  6. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid
    #140893
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
    Depositor
    Yasushi Okamura
    Article
    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1
    Insert
    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Zebrafish, Ciona intestinalis)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…
    Promoter
    Available since
    May 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    140893-plasmid-map-sequence-id-297104

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  7. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79887-plasmid-map-sequence-id-402553

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  13. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    flk1
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  14. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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  15. pMT-ubb-Avi-Cerulean-RanGAP

    Plasmid
    #79881
    Purpose
    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79881-plasmid-map-sequence-id-149777

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  17. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  18. pTol2-PcyA-IRES-HO1

    Plasmid
    #131787
    Purpose
    Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.
    Depositor
    George Eisenhoffer
    Article
    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi
    Insert
    PcyA and HO1
    Use
    Synthetic Biology; Zebrafish tol2 transposon
    Tags
    MTS (Mitochondrial Targeting Signal from subunit …
    Expression
    Mammalian
    Mutation
    Promoter
    hsp70
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    131787-plasmid-map-sequence-id-268074

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  20. Danio alpha Bb-crystallin 1kb promoter/AcGFP

    Plasmid
    #98098
    Purpose
    aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio aBb-crystallin 1 kb fragment (-1074/-1)
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98098-plasmid-map-sequence-id-193846

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Showing: 2361 - 2380 of 3119 results