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pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

Plasmid

#101064

Purpose

AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2_L398T

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBBRBB-eGFP

Plasmid

#32549

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pLY093-EFS-BCMA-Blast-WPRE

Plasmid

#192194

Purpose

Lenti-BCMA-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCRZero

Plasmid

#120275

Purpose

Blunt-end cloning vector of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Article

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only
pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

Plasmid

#201681

Purpose

Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

Depositor

Emma Rawlins

Article

Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

Inserts

SFTPC promoter region (2kb upstream)
TagRFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Blast

Plasmid

#111887

Purpose

Lentiviral expression of cDNA with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi-FoxO1_1R_10A_3D

Plasmid

#106278

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Use

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Deleted amino acids 401-636, S209A, H212R, S215A,…

Promoter

EF1a/RPBSA

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-DRGFP

Plasmid

#113193

Purpose

DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

Depositor

Adam Karpf

Insert

DR-GFP

Use

Donor construct with t2a-hygromycin for gene trap…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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